5wx9
From Proteopedia
(Difference between revisions)
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<StructureSection load='5wx9' size='340' side='right'caption='[[5wx9]], [[Resolution|resolution]] 1.76Å' scene=''> | <StructureSection load='5wx9' size='340' side='right'caption='[[5wx9]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5wx9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WX9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WX9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WX9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WX9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wx9 OCA], [http://pdbe.org/5wx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wx9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wx9 ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERF096, At5g43410, MWF20.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wx9 OCA], [http://pdbe.org/5wx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wx9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wx9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/ERF96_ARATH ERF96_ARATH]] Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). | [[http://www.uniprot.org/uniprot/ERF96_ARATH ERF96_ARATH]] Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal alpha-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal alpha-helix and AP2/ERF domain. | ||
| + | |||
| + | Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box.,Chen CY, Lin PH, Chen KH, Cheng YS Plant Mol Biol. 2020 Nov;104(4-5):483-498. doi: 10.1007/s11103-020-01052-5. Epub , 2020 Aug 19. PMID:32813232<ref>PMID:32813232</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5wx9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arath]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chen, C Y]] | [[Category: Chen, C Y]] | ||
Revision as of 09:44, 18 November 2020
Crystal Structure of AtERF96 with GCC-box
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