6xr8
From Proteopedia
(Difference between revisions)
m (Protected "6xr8" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
==Distinct conformational states of SARS-CoV-2 spike protein== | ==Distinct conformational states of SARS-CoV-2 spike protein== | ||
| - | <StructureSection load='6xr8' size='340' side='right'caption='[[6xr8]]' scene=''> | + | <StructureSection load='6xr8' size='340' side='right'caption='[[6xr8]], [[Resolution|resolution]] 2.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XR8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XR8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xr8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XR8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XR8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xr8 OCA], [http://pdbe.org/6xr8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xr8 RCSB], [http://www.ebi.ac.uk/pdbsum/6xr8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xr8 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S, 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xr8 OCA], [http://pdbe.org/6xr8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xr8 RCSB], [http://www.ebi.ac.uk/pdbsum/6xr8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xr8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2]] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] | ||
| + | |||
| + | ==See Also== | ||
| + | *[[SARS-CoV-2 spike protein fusion transformation|SARS-CoV-2 spike protein fusion transformation]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: 2019-ncov]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cai | + | [[Category: Cai, Y F]] |
| - | [[Category: Chen B]] | + | [[Category: Chen, B]] |
| - | [[Category: Peng | + | [[Category: Jr, R M.Walsh]] |
| - | [[Category: Rawson S]] | + | [[Category: Peng, H Q]] |
| - | [[Category: Sterling | + | [[Category: Rawson, S]] |
| - | [[Category: Volloch | + | [[Category: Sterling, S M]] |
| - | [[Category: | + | [[Category: Volloch, S R]] |
| - | [[Category: | + | [[Category: Xiao, T S]] |
| - | [[Category: | + | [[Category: Zhang, J]] |
| + | [[Category: Viral protein]] | ||
Revision as of 09:54, 18 November 2020
Distinct conformational states of SARS-CoV-2 spike protein
| |||||||||||
Categories: 2019-ncov | Large Structures | Cai, Y F | Chen, B | Jr, R M.Walsh | Peng, H Q | Rawson, S | Sterling, S M | Volloch, S R | Xiao, T S | Zhang, J | Viral protein
