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1h87

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Revision as of 04:35, 19 November 2020

Gadolinium derivative of tetragonal Hen Egg-White Lysozyme at 1.7 A resolution

Structural highlights

1h87 is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	132l, 193l, 194l, 1a2y, 1aki, 1at5, 1at6, 1azf, 1b0d, 1b2k, 1bgi, 1bhz, 1bvk, 1bvx, 1bwh, 1bwi, 1bwj, 1c08, 1c10, 1dpw, 1dpx, 1dqj, 1e8l, 1f0w, 1f10, 1f3j, 1fdl, 1flq, 1flu, 1flw, 1fly, 1fn5, 1g7h, 1g7i, 1g7j, 1g7l, 1g7m, 1hel, 1hem, 1hen, 1heo, 1hep, 1heq, 1her, 1hew, 1hf4, 1hsw, 1hsx, 1jpo, 1kip, 1kiq, 1kir, 1kxw, 1kxx, 1kxy, 1lcn, 1lkr, 1lks, 1lma, 1lpi, 1lsa, 1lsb, 1lsc, 1lsd, 1lse, 1lsf, 1lsg, 1lsm, 1lsn, 1lsy, 1lsz, 1lyo, 1lys, 1lyz, 1lz8, 1lza, 1lzb, 1lzc, 1lzd, 1lze, 1lzg, 1lzh, 1lzn, 1lzt, 1mel, 1mlc, 1qtk, 1rcm, 1rfp, 1uco, 1uia, 1uib, 1uic, 1uid, 1uie, 1uif, 1uig, 1uih, 1xei, 1xej, 1xek, 2hfm, 2iff, 2lym, 2lyo, 2lyz, 2lzh, 2lzt, 3hfl, 3hfm, 3lym, 3lyo, 3lyt, 3lyz, 3lzt, 4lym, 4lyo, 4lyt, 4lyz, 4lzt, 5lym, 5lyt, 5lyz, 6lyt, 6lyz, 7lyz, 8lyz
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A neutral gadolinium complex, Gd-HPDO3A, is shown to be a good candidate to use to obtain heavy-atom derivatives and solve macromolecular structures using anomalous dispersion. Tetragonal crystals of a gadolinium derivative of hen egg-white lysozyme were obtained by co-crystallization using different concentrations of the complex. Diffraction data from three derivative crystals (100, 50 and 10 mM) were collected to a resolution of 1.7 A using Cu Kalpha radiation from a rotating anode. Two strong binding sites of the gadolinium complex to the protein were located from the gadolinium anomalous signal in both the 100 and 50 mM derivatives. A single site is occupied in the 10 mM derivative. Phasing using the anomalous signal at a single wavelength (SAD method) leads to an electron-density map of high quality. The structure of the 100 mM derivative has been refined. Two molecules of the gadolinium complex are close together. Both molecules are located close to tryptophan residues. Four chloride ions were found. The exceptional quality of the SAD electron-density map, only enhanced by solvent flattening, suggests that single-wavelength anomalous scattering with the Gd-HPDO3A complex may be sufficient to solve protein structures of high molecular weight by synchrotron-radiation experiments, if not by laboratory experiments.

Gd-HPDO3A, a complex to obtain high-phasing-power heavy-atom derivatives for SAD and MAD experiments: results with tetragonal hen egg-white lysozyme.,Girard E, Chantalat L, Vicat J, Kahn R Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):1-9. Epub 2001 Dec, 21. PMID:11752774^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Girard E, Chantalat L, Vicat J, Kahn R. Gd-HPDO3A, a complex to obtain high-phasing-power heavy-atom derivatives for SAD and MAD experiments: results with tetragonal hen egg-white lysozyme. Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):1-9. Epub 2001 Dec, 21. PMID:11752774

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h87"

@@ Line 1: / Line 1: @@
 ==Gadolinium derivative of tetragonal Hen Egg-White Lysozyme at 1.7 A resolution==
-<StructureSection load='1h87' size='340' side='right'  caption='[[1h87]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
+<StructureSection load='1h87' size='340' side='right'caption='[[1h87]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1h87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H87 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1h87]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H87 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1H87 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DO3:10-((2R)-2-HYDROXYPROPYL)-1,4,7,10-TETRAAZACYCLODODECANE+1,4,7-TRIACETIC+ACID'>DO3</scene>, <scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DO3:10-((2R)-2-HYDROXYPROPYL)-1,4,7,10-TETRAAZACYCLODODECANE+1,4,7-TRIACETIC+ACID'>DO3</scene>, <scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[132l|132l]], [[193l|193l]], [[194l|194l]], [[1a2y|1a2y]], [[1aki|1aki]], [[1at5|1at5]], [[1at6|1at6]], [[1azf|1azf]], [[1b0d|1b0d]], [[1b2k|1b2k]], [[1bgi|1bgi]], [[1bhz|1bhz]], [[1bvk|1bvk]], [[1bvx|1bvx]], [[1bwh|1bwh]], [[1bwi|1bwi]], [[1bwj|1bwj]], [[1c08|1c08]], [[1c10|1c10]], [[1dpw|1dpw]], [[1dpx|1dpx]], [[1dqj|1dqj]], [[1e8l|1e8l]], [[1f0w|1f0w]], [[1f10|1f10]], [[1f3j|1f3j]], [[1fdl|1fdl]], [[1flq|1flq]], [[1flu|1flu]], [[1flw|1flw]], [[1fly|1fly]], [[1fn5|1fn5]], [[1g7h|1g7h]], [[1g7i|1g7i]], [[1g7j|1g7j]], [[1g7l|1g7l]], [[1g7m|1g7m]], [[1hel|1hel]], [[1hem|1hem]], [[1hen|1hen]], [[1heo|1heo]], [[1hep|1hep]], [[1heq|1heq]], [[1her|1her]], [[1hew|1hew]], [[1hf4|1hf4]], [[1hsw|1hsw]], [[1hsx|1hsx]], [[1jpo|1jpo]], [[1kip|1kip]], [[1kiq|1kiq]], [[1kir|1kir]], [[1kxw|1kxw]], [[1kxx|1kxx]], [[1kxy|1kxy]], [[1lcn|1lcn]], [[1lkr|1lkr]], [[1lks|1lks]], [[1lma|1lma]], [[1lpi|1lpi]], [[1lsa|1lsa]], [[1lsb|1lsb]], [[1lsc|1lsc]], [[1lsd|1lsd]], [[1lse|1lse]], [[1lsf|1lsf]], [[1lsg|1lsg]], [[1lsm|1lsm]], [[1lsn|1lsn]], [[1lsy|1lsy]], [[1lsz|1lsz]], [[1lyo|1lyo]], [[1lys|1lys]], [[1lyz|1lyz]], [[1lz8|1lz8]], [[1lza|1lza]], [[1lzb|1lzb]], [[1lzc|1lzc]], [[1lzd|1lzd]], [[1lze|1lze]], [[1lzg|1lzg]], [[1lzh|1lzh]], [[1lzn|1lzn]], [[1lzt|1lzt]], [[1mel|1mel]], [[1mlc|1mlc]], [[1qtk|1qtk]], [[1rcm|1rcm]], [[1rfp|1rfp]], [[1uco|1uco]], [[1uia|1uia]], [[1uib|1uib]], [[1uic|1uic]], [[1uid|1uid]], [[1uie|1uie]], [[1uif|1uif]], [[1uig|1uig]], [[1uih|1uih]], [[1xei|1xei]], [[1xej|1xej]], [[1xek|1xek]], [[2hfm|2hfm]], [[2iff|2iff]], [[2lym|2lym]], [[2lyo|2lyo]], [[2lyz|2lyz]], [[2lzh|2lzh]], [[2lzt|2lzt]], [[3hfl|3hfl]], [[3hfm|3hfm]], [[3lym|3lym]], [[3lyo|3lyo]], [[3lyt|3lyt]], [[3lyz|3lyz]], [[3lzt|3lzt]], [[4lym|4lym]], [[4lyo|4lyo]], [[4lyt|4lyt]], [[4lyz|4lyz]], [[4lzt|4lzt]], [[5lym|5lym]], [[5lyt|5lyt]], [[5lyz|5lyz]], [[6lyt|6lyt]], [[6lyz|6lyz]], [[7lyz|7lyz]], [[8lyz|8lyz]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[132l|132l]], [[193l|193l]], [[194l|194l]], [[1a2y|1a2y]], [[1aki|1aki]], [[1at5|1at5]], [[1at6|1at6]], [[1azf|1azf]], [[1b0d|1b0d]], [[1b2k|1b2k]], [[1bgi|1bgi]], [[1bhz|1bhz]], [[1bvk|1bvk]], [[1bvx|1bvx]], [[1bwh|1bwh]], [[1bwi|1bwi]], [[1bwj|1bwj]], [[1c08|1c08]], [[1c10|1c10]], [[1dpw|1dpw]], [[1dpx|1dpx]], [[1dqj|1dqj]], [[1e8l|1e8l]], [[1f0w|1f0w]], [[1f10|1f10]], [[1f3j|1f3j]], [[1fdl|1fdl]], [[1flq|1flq]], [[1flu|1flu]], [[1flw|1flw]], [[1fly|1fly]], [[1fn5|1fn5]], [[1g7h|1g7h]], [[1g7i|1g7i]], [[1g7j|1g7j]], [[1g7l|1g7l]], [[1g7m|1g7m]], [[1hel|1hel]], [[1hem|1hem]], [[1hen|1hen]], [[1heo|1heo]], [[1hep|1hep]], [[1heq|1heq]], [[1her|1her]], [[1hew|1hew]], [[1hf4|1hf4]], [[1hsw|1hsw]], [[1hsx|1hsx]], [[1jpo|1jpo]], [[1kip|1kip]], [[1kiq|1kiq]], [[1kir|1kir]], [[1kxw|1kxw]], [[1kxx|1kxx]], [[1kxy|1kxy]], [[1lcn|1lcn]], [[1lkr|1lkr]], [[1lks|1lks]], [[1lma|1lma]], [[1lpi|1lpi]], [[1lsa|1lsa]], [[1lsb|1lsb]], [[1lsc|1lsc]], [[1lsd|1lsd]], [[1lse|1lse]], [[1lsf|1lsf]], [[1lsg|1lsg]], [[1lsm|1lsm]], [[1lsn|1lsn]], [[1lsy|1lsy]], [[1lsz|1lsz]], [[1lyo|1lyo]], [[1lys|1lys]], [[1lyz|1lyz]], [[1lz8|1lz8]], [[1lza|1lza]], [[1lzb|1lzb]], [[1lzc|1lzc]], [[1lzd|1lzd]], [[1lze|1lze]], [[1lzg|1lzg]], [[1lzh|1lzh]], [[1lzn|1lzn]], [[1lzt|1lzt]], [[1mel|1mel]], [[1mlc|1mlc]], [[1qtk|1qtk]], [[1rcm|1rcm]], [[1rfp|1rfp]], [[1uco|1uco]], [[1uia|1uia]], [[1uib|1uib]], [[1uic|1uic]], [[1uid|1uid]], [[1uie|1uie]], [[1uif|1uif]], [[1uig|1uig]], [[1uih|1uih]], [[1xei|1xei]], [[1xej|1xej]], [[1xek|1xek]], [[2hfm|2hfm]], [[2iff|2iff]], [[2lym|2lym]], [[2lyo|2lyo]], [[2lyz|2lyz]], [[2lzh|2lzh]], [[2lzt|2lzt]], [[3hfl|3hfl]], [[3hfm|3hfm]], [[3lym|3lym]], [[3lyo|3lyo]], [[3lyt|3lyt]], [[3lyz|3lyz]], [[3lzt|3lzt]], [[4lym|4lym]], [[4lyo|4lyo]], [[4lyt|4lyt]], [[4lyz|4lyz]], [[4lzt|4lzt]], [[5lym|5lym]], [[5lyt|5lyt]], [[5lyz|5lyz]], [[6lyt|6lyt]], [[6lyz|6lyz]], [[7lyz|7lyz]], [[8lyz|8lyz]]</div></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h87 OCA], [http://pdbe.org/1h87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h87 RCSB], [http://www.ebi.ac.uk/pdbsum/1h87 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h87 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1h87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h87 OCA], [http://pdbe.org/1h87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h87 RCSB], [http://www.ebi.ac.uk/pdbsum/1h87 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h87 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 38: / Line 38: @@
 </StructureSection>
 [[Category: Gallus gallus]]
+[[Category: Large Structures]]
 [[Category: Lysozyme]]
 [[Category: Chantalat, L]]

1h87

From Proteopedia

Revision as of 04:35, 19 November 2020

Gadolinium derivative of tetragonal Hen Egg-White Lysozyme at 1.7 A resolution

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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