1lu9

From Proteopedia

(Difference between revisions)

Revision as of 08:02, 25 November 2020

Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1

Structural highlights

1lu9 is a 3 chain structure with sequence from Metea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1lua
Gene:	mtdA (METEA)
Activity:	Methylenetetrahydrofolate dehydrogenase (NADP(+)), with EC number 1.5.1.5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MTDA_METEA] Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

NADP-dependent methylene-H(4)MPT dehydrogenase, MtdA, from Methylobacterium extorquens AM1 catalyzes the dehydrogenation of methylene-tetrahydromethanopterin and methylene-tetrahydrofolate with NADP(+) as cosubstrate. The X-ray structure of MtdA with and without NADP bound was established at 1.9 A resolution. The enzyme is present as a homotrimer. The alpha,beta fold of the monomer is related to that of methylene-H(4)F dehydrogenases, suggesting a common evolutionary origin. The position of the active site is located within a large crevice built up by the two domains of one subunit and one domain of a second subunit. Methylene-H(4)MPT could be modeled into the cleft, and crucial active site residues such as Phe18, Lys256, His260, and Thr102 were identified. The molecular basis of the different substrate specificities and different catalytic demands of MtdA compared to methylene-H(4)F dehydrogenases are discussed.

Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1.,Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA Structure. 2002 Aug;10(8):1127-37. PMID:12176390^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ermler U, Hagemeier CH, Roth A, Demmer U, Grabarse W, Warkentin E, Vorholt JA. Structure of methylene-tetrahydromethanopterin dehydrogenase from methylobacterium extorquens AM1. Structure. 2002 Aug;10(8):1127-37. PMID:12176390

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lu9"

@@ Line 1: / Line 1: @@
 ==Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1==
-<StructureSection load='1lu9' size='340' side='right' caption='[[1lu9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='1lu9' size='340' side='right'caption='[[1lu9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1lu9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Metea Metea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LU9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1lu9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Metea Metea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LU9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1LU9 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lua|1lua]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lua|1lua]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mtdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272630 METEA])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_dehydrogenase_(NADP(+)) Methylenetetrahydrofolate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.5 1.5.1.5] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lu9 OCA], [http://pdbe.org/1lu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lu9 RCSB], [http://www.ebi.ac.uk/pdbsum/1lu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lu9 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1lu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lu9 OCA], [http://pdbe.org/1lu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lu9 RCSB], [http://www.ebi.ac.uk/pdbsum/1lu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lu9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 34: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Metea]]
 [[Category: Demmer, U]]

1lu9

From Proteopedia

Revision as of 08:02, 25 November 2020

Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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