1d6k
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1d6k.gif|left|200px]] | [[Image:1d6k.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1d6k", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1d6k| PDB=1d6k | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX''' | '''NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX''' | ||
| Line 30: | Line 27: | ||
[[Category: Stoldt, M.]] | [[Category: Stoldt, M.]] | ||
[[Category: Wohnert, J.]] | [[Category: Wohnert, J.]] | ||
| - | [[Category: | + | [[Category: Protein-rna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:30:25 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:30, 2 May 2008
NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX
Overview
The structure of the complex between ribosomal protein L25 and a 37 nucleotide RNA molecule, which contains the E-loop and helix IV regions of the E-domain of Escherichia coli 5S rRNA, has been determined to an overall r.m.s. displacement of 1.08 A (backbone heavy atoms) by heteronuclear NMR spectroscopy (Protein Databank code 1d6k). The interacting molecular surfaces are bipartite for both the RNA and the protein. One side of the six-stranded beta-barrel of L25 recognizes the minor groove of the E-loop with very little change in the conformations of either the protein or the RNA and with the RNA-protein interactions occurring mainly along one strand of the E-loop duplex. This minor groove recognition module includes two parallel beta-strands of L25, a hitherto unknown RNA binding topology. Binding of the RNA also induces conversion of a flexible loop to an alpha-helix in L25, the N-terminal tip of which interacts with the widened major groove at the E-loop/helix IV junction of the RNA. The structure of the complex reveals that the E-domain RNA serves as a preformed docking partner, while the L25 protein has one preformed and one induced recognition module.
About this Structure
1D6K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition., Stoldt M, Wohnert J, Ohlenschlager O, Gorlach M, Brown LR, EMBO J. 1999 Nov 15;18(22):6508-21. PMID:10562563 Page seeded by OCA on Fri May 2 13:30:25 2008
