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1m7w

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Revision as of 08:34, 2 December 2020

HNF4a ligand binding domain with bound fatty acid

Structural highlights

1m7w is a 4 chain structure with sequence from Black rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	HNF4a (Black rat)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HNF4A_RAT] Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

HNF4 alpha is an orphan member of the nuclear receptor family with prominent functions in liver, gut, kidney and pancreatic beta cells. We have solved the x-ray crystal structure of the HNF4 alpha ligand binding domain, which adopts a canonical fold. Two conformational states are present within each homodimer: an open form with alpha helix 12 (alpha 12) extended and collinear with alpha 10 and a closed form with alpha 12 folded against the body of the domain. Although the protein was crystallized without added ligands, the ligand binding pockets of both closed and open forms contain fatty acids. The carboxylic acid headgroup of the fatty acid ion pairs with the guanidinium group of Arg(226) at one end of the ligand binding pocket, while the aliphatic chain fills a long, narrow channel that is lined with hydrophobic residues. These findings suggest that fatty acids are endogenous ligands for HNF4 alpha and establish a framework for understanding how HNF4 alpha activity is enhanced by ligand binding and diminished by MODY1 mutations.

Crystal structure of the HNF4 alpha ligand binding domain in complex with endogenous fatty acid ligand.,Dhe-Paganon S, Duda K, Iwamoto M, Chi YI, Shoelson SE J Biol Chem. 2002 Oct 11;277(41):37973-6. Epub 2002 Aug 21. PMID:12193589^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dhe-Paganon S, Duda K, Iwamoto M, Chi YI, Shoelson SE. Crystal structure of the HNF4 alpha ligand binding domain in complex with endogenous fatty acid ligand. J Biol Chem. 2002 Oct 11;277(41):37973-6. Epub 2002 Aug 21. PMID:12193589 doi:10.1074/jbc.C200420200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1m7w"

@@ Line 1: / Line 1: @@
 ==HNF4a ligand binding domain with bound fatty acid==
-<StructureSection load='1m7w' size='340' side='right' caption='[[1m7w]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='1m7w' size='340' side='right'caption='[[1m7w]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1m7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Black_rat Black rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M7W FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1m7w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Black_rat Black rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1M7W FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HNF4a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10117 Black rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7w OCA], [http://pdbe.org/1m7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m7w RCSB], [http://www.ebi.ac.uk/pdbsum/1m7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7w ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1m7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7w OCA], [http://pdbe.org/1m7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m7w RCSB], [http://www.ebi.ac.uk/pdbsum/1m7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7w ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 34: @@
 </StructureSection>
 [[Category: Black rat]]
+[[Category: Large Structures]]
 [[Category: Chi, Y I]]
 [[Category: Dhe-Paganon, S]]

1m7w

From Proteopedia

Revision as of 08:34, 2 December 2020

HNF4a ligand binding domain with bound fatty acid

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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