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1mvf

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Revision as of 08:41, 2 December 2020

MazE addiction antidote

Structural highlights

1mvf is a 4 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895 and Arabian camel. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	MazE ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MAZE_ECOLI] Antitoxin component of a toxin-antitoxin (TA) module. Labile antitoxin that binds to the MazF mRNA interferase toxin and neutralizes its endoribonuclease activity. Is considered to be an 'addiction' molecule as the cell will die in its absence. The endoribonuclease activity (MazF, a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. This effect can be rescued by expression of MazE, but after 6 hours in rich medium the overexpression of MazF leads to programmed cell death. Cell growth and viability are not affected when MazF and MazE are coexpressed. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription. Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation, while MazE-MazF is also implicated in cell death in liquid media.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A specific camel VHH (variable domain of dromedary heavy chain antibody) fragment was used to crystallize the intrinsically flexible addiction antidote MazE. Only 45% of the polypeptide chain is found ordered in the crystal. The MazE monomer consisting of two beta-hairpins connected by a short alpha-helix has no hydrophobic core on its own and represents only one half of a typical protein domain. A complete domain structure is formed by the association of two chains, creating a hydrophobic core between two four-stranded beta-sheets. This hydrophobic core consists exclusively of short aliphatic residues. The folded part of MazE contains a novel DNA binding motif. A model for DNA binding that is consistent with the available biochemical data is presented.

Crystal structure of the intrinsically flexible addiction antidote MazE.,Loris R, Marianovsky I, Lah J, Laeremans T, Engelberg-Kulka H, Glaser G, Muyldermans S, Wyns L J Biol Chem. 2003 Jul 25;278(30):28252-7. Epub 2003 May 12. PMID:12743116^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Loris R, Marianovsky I, Lah J, Laeremans T, Engelberg-Kulka H, Glaser G, Muyldermans S, Wyns L. Crystal structure of the intrinsically flexible addiction antidote MazE. J Biol Chem. 2003 Jul 25;278(30):28252-7. Epub 2003 May 12. PMID:12743116 doi:10.1074/jbc.M302336200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mvf"

@@ Line 1: / Line 1: @@
 ==MazE addiction antidote==
-<StructureSection load='1mvf' size='340' side='right' caption='[[1mvf]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='1mvf' size='340' side='right'caption='[[1mvf]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1mvf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1mvf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MVF FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MazE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvf OCA], [http://pdbe.org/1mvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mvf RCSB], [http://www.ebi.ac.uk/pdbsum/1mvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvf ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvf OCA], [http://pdbe.org/1mvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mvf RCSB], [http://www.ebi.ac.uk/pdbsum/1mvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvf ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 34: / Line 34: @@
 [[Category: Bacillus coli migula 1895]]
 [[Category: Arabian camel]]
+[[Category: Large Structures]]
 [[Category: Engelberg-Kulka, H]]
 [[Category: Glaser, G]]

1mvf

From Proteopedia

Revision as of 08:41, 2 December 2020

MazE addiction antidote

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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