1d7m
From Proteopedia
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'''COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I''' | '''COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I''' | ||
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[[Category: Kammerer, R A.]] | [[Category: Kammerer, R A.]] | ||
[[Category: Steinmetz, M O.]] | [[Category: Steinmetz, M O.]] | ||
| - | [[Category: | + | [[Category: Alpha helix]] |
| - | [[Category: | + | [[Category: Coiled-coil]] |
| - | [[Category: | + | [[Category: Coiled-coil trigger site]] |
| - | [[Category: | + | [[Category: Dimerization]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:32:11 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:32, 2 May 2008
COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I
Overview
BACKGROUND: The parallel two-stranded alpha-helical coiled coil is the most frequently encountered subunit-oligomerization motif in proteins. The simplicity and regularity of this motif have made it an attractive system to explore some of the fundamental principles of protein folding and stability and to test the principles of de novo design. RESULTS: The X-ray crystal structure of the 18-heptad-repeat alpha-helical coiled-coil domain of the actin-bundling protein cortexillin I from Dictyostelium discoideum is a tightly packed parallel two-stranded alpha-helical coiled coil. It harbors a distinct 14-residue sequence motif that is essential for coiled-coil formation, and is a prerequisite for the assembly of cortexillin I. The atomic structure reveals novel types of ionic coiled-coil interactions. In particular, the structure shows that a characteristic interhelical and intrahelical salt-bridge pattern, in combination with the hydrophobic interactions occurring at the dimer interface, is the key structural feature of its coiled-coil trigger site. CONCLUSIONS: The knowledge gained from the structure could be used in the de novo design of alpha-helical coiled coils for applications such as two-stage drug targeting and delivery systems, and in the design of coiled coils as templates for combinatorial helical libraries in drug discovery and as synthetic carrier molecules.
About this Structure
1D7M is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.
Reference
The coiled-coil trigger site of the rod domain of cortexillin I unveils a distinct network of interhelical and intrahelical salt bridges., Burkhard P, Kammerer RA, Steinmetz MO, Bourenkov GP, Aebi U, Structure. 2000 Mar 15;8(3):223-30. PMID:10745004 Page seeded by OCA on Fri May 2 13:32:11 2008
