2-isopropylmalate synthase

Function

2-isopropylmalate synthase (LeuA) or α-isopropylmalate synthase catalyzes the reversible conversion of 3-methyl-2-oxobutanoate and acetyl-CoA to 2-isopropylmalate. LeuA participates in the biosynthesis of leucine and pyruvate metabolism^[1].

Structural highlights

. LeuA structure is composed of 2 major domains - the catalytic N-terminal which shows a TIM barrel conformation and the regulatory C-terminal. The 2 domains are separated by subdomains I and II and a short flexible hinge region between the 2 subdomains. The catalytic domain binds the substrate and the ^[2]. Water molecules are shown as red spheres.

3D structures of 2-isopropylmalate synthase

2-isopropylmalate synthase 3D structures