1d88

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[[Image:1d88.gif|left|200px]]
[[Image:1d88.gif|left|200px]]
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{{Structure
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|PDB= 1d88 |SIZE=350|CAPTION= <scene name='initialview01'>1d88</scene>, resolution 2.000&Aring;
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The line below this paragraph, containing "STRUCTURE_1d88", creates the "Structure Box" on the page.
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{{STRUCTURE_1d88| PDB=1d88 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d88 OCA], [http://www.ebi.ac.uk/pdbsum/1d88 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d88 RCSB]</span>
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'''CONFORMATIONAL INFLUENCE OF THE RIBOSE 2'-HYDROXYL GROUP: CRYSTAL STRUCTURES OF DNA-RNA CHIMERIC DUPLEXES'''
'''CONFORMATIONAL INFLUENCE OF THE RIBOSE 2'-HYDROXYL GROUP: CRYSTAL STRUCTURES OF DNA-RNA CHIMERIC DUPLEXES'''
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==About this Structure==
==About this Structure==
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1D88 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D88 OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D88 OCA].
==Reference==
==Reference==
Conformational influence of the ribose 2'-hydroxyl group: crystal structures of DNA-RNA chimeric duplexes., Egli M, Usman N, Rich A, Biochemistry. 1993 Apr 6;32(13):3221-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7681688 7681688]
Conformational influence of the ribose 2'-hydroxyl group: crystal structures of DNA-RNA chimeric duplexes., Egli M, Usman N, Rich A, Biochemistry. 1993 Apr 6;32(13):3221-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7681688 7681688]
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[[Category: Protein complex]]
 
[[Category: Egli, M.]]
[[Category: Egli, M.]]
[[Category: Rich, A.]]
[[Category: Rich, A.]]
[[Category: Usman, N.]]
[[Category: Usman, N.]]
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[[Category: a-dna/rna]]
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[[Category: A-dna/rna]]
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[[Category: double helix]]
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[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:33:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:36:36 2008''
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Revision as of 10:33, 2 May 2008

Image:1d88.gif

Template:STRUCTURE 1d88

CONFORMATIONAL INFLUENCE OF THE RIBOSE 2'-HYDROXYL GROUP: CRYSTAL STRUCTURES OF DNA-RNA CHIMERIC DUPLEXES


Overview

We have crystallized three double-helical DNA-RNA chimeric duplexes and determined their structures by X-ray crystallography at resolutions between 2 and 2.25 A. The two self-complementary duplexes [r(G)d(CGTATACGC)]2 and [d(GCGT)r(A)d(TACGC)]2, as well as the Okazaki fragment d(GGGTATACGC).r(GCG)d(TATACCC), were found to adopt A-type conformations. The crystal structures are non-isomorphous, and the crystallographic environments for the three chimeras are different. A number of intramolecular interactions of the ribose 2'-hydroxyl groups contribute to the stabilization of the A-conformation. Hydrogen bonds between 2'-hydroxyls and 5'-oxygens or phosphate oxygens, in addition to the previously observed hydrogen bonds to 1'-oxygens of adjacent riboses and deoxyriboses, are observed in the DNA-RNA chimeric duplexes. The crystalline chimeric duplexes do not show a transition between the DNA A- and B-conformations. CD spectra suggest that the Okazaki fragment assumes an A-conformation in solution as well. In this molecule the three RNA residues may therefore lock the complete decamer in the A-conformation. Crystals of an all-DNA strand with the same sequence as the self-complementary chimeras show a morphology which is different from those of the chimera crystals. Moreover, the oligonucleotide does not match any of the sequence characteristics of DNAs usually adopting the A-conformation in the crystalline state (e.g., octamers with short alternating stretches of purines and pyrimidines). In DNA-RNA chimeric duplexes, it is therefore possible that a single RNA residue can drive the conformational equilibrium toward the A-conformation.

About this Structure

Full crystallographic information is available from OCA.

Reference

Conformational influence of the ribose 2'-hydroxyl group: crystal structures of DNA-RNA chimeric duplexes., Egli M, Usman N, Rich A, Biochemistry. 1993 Apr 6;32(13):3221-37. PMID:7681688 Page seeded by OCA on Fri May 2 13:33:34 2008

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