1d91
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1d91.gif|left|200px]] | [[Image:1d91.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1d91", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1d91| PDB=1d91 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)''' | '''G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D91 OCA]. | |
==Reference== | ==Reference== | ||
G . T base-pairs in a DNA helix: the crystal structure of d(G-G-G-G-T-C-C-C)., Kneale G, Brown T, Kennard O, Rabinovich D, J Mol Biol. 1985 Dec 20;186(4):805-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/4093986 4093986] | G . T base-pairs in a DNA helix: the crystal structure of d(G-G-G-G-T-C-C-C)., Kneale G, Brown T, Kennard O, Rabinovich D, J Mol Biol. 1985 Dec 20;186(4):805-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/4093986 4093986] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Brown, T.]] | [[Category: Brown, T.]] | ||
[[Category: Kennard, O.]] | [[Category: Kennard, O.]] | ||
[[Category: Kneale, G.]] | [[Category: Kneale, G.]] | ||
[[Category: Rabinovich, D.]] | [[Category: Rabinovich, D.]] | ||
| - | [[Category: | + | [[Category: A-dna]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | [[Category: | + | [[Category: Mismatched]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:35:11 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:35, 2 May 2008
G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)
Overview
The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an A-type DNA double helix containing two adjacent G . T base-pair mismatches. The structure has been refined to an R-factor of 14% at 2.1 A resolution with 104 solvent molecules located. The two G . T mismatches adopt the "wobble" form of base-pairing. The mismatched bases are linked by a network of water molecules interacting with the exposed functional groups in both the major and minor grooves. The presence of two mispaired bases in the octamer has surprisingly little effect on the global structure of the helix or the backbone and glycosidic torsional angles. Base stacking around the mismatch is perturbed, but the central G-T step shows particularly good base overlap, which may contribute to the relatively high stability of this oligomer.
About this Structure
Full crystallographic information is available from OCA.
Reference
G . T base-pairs in a DNA helix: the crystal structure of d(G-G-G-G-T-C-C-C)., Kneale G, Brown T, Kennard O, Rabinovich D, J Mol Biol. 1985 Dec 20;186(4):805-14. PMID:4093986 Page seeded by OCA on Fri May 2 13:35:11 2008
