6m55
From Proteopedia
(Difference between revisions)
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==Crystal structure of the E496A mutant of HsBglA in complex with 4-galactosyllactose== | ==Crystal structure of the E496A mutant of HsBglA in complex with 4-galactosyllactose== | ||
| - | <StructureSection load='6m55' size='340' side='right'caption='[[6m55]]' scene=''> | + | <StructureSection load='6m55' size='340' side='right'caption='[[6m55]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M55 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M55 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m55]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24193 Atcc 24193]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M55 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M55 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m55 OCA], [http://pdbe.org/6m55 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m55 RCSB], [http://www.ebi.ac.uk/pdbsum/6m55 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m55 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bglA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5445 ATCC 24193])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m55 OCA], [http://pdbe.org/6m55 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m55 RCSB], [http://www.ebi.ac.uk/pdbsum/6m55 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m55 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A GH1 beta-glucosidase from the fungus Hamamotoa singularis (HsBglA) has high transgalactosylation activity and efficiently converts lactose to galactooligosaccharides. Consequently, HsBglA is among the most widely used enzymes for industrial galactooligosaccharide production. Here, we present the first crystal structures of HsBglA with and without 4'-galactosyllactose, a tri-galactooligosaccharide, at 3.0 and 2.1 A resolutions, respectively. These structures reveal details of the structural elements that define the catalytic activity and substrate binding of HsBglA, and provide a possible interpretation for its high catalytic potency for transgalactosylation reaction. | ||
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| + | Crystal structure of a GH1 beta-glucosidase from Hamamotoa singularis.,Uehara R, Iwamoto R, Aoki S, Yoshizawa T, Takano K, Matsumura H, Tanaka SI Protein Sci. 2020 Jul 26. doi: 10.1002/pro.3916. PMID:32713015<ref>PMID:32713015</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6m55" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 24193]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Aoki S]] | + | [[Category: Aoki, S]] |
| - | [[Category: Iwamoto R]] | + | [[Category: Iwamoto, R]] |
| - | [[Category: Matsumura H]] | + | [[Category: Matsumura, H]] |
| - | [[Category: Takano K]] | + | [[Category: Takano, K]] |
| - | [[Category: Tanaka S | + | [[Category: Tanaka, S i]] |
| - | [[Category: Uehara R]] | + | [[Category: Uehara, R]] |
| - | [[Category: Yoshizawa T]] | + | [[Category: Yoshizawa, T]] |
| + | [[Category: Beta-galactosidase]] | ||
| + | [[Category: Beta-glucosidase]] | ||
| + | [[Category: Galactooligosaccharide]] | ||
| + | [[Category: Hamamotoa singulari]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Structural protein]] | ||
| + | [[Category: Transgalactosylation]] | ||
Revision as of 12:42, 16 December 2020
Crystal structure of the E496A mutant of HsBglA in complex with 4-galactosyllactose
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