6wkw
From Proteopedia
(Difference between revisions)
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==EM structure of CtBP2 with a minimal dehydrogenase domain of CtBP2== | ==EM structure of CtBP2 with a minimal dehydrogenase domain of CtBP2== | ||
| - | <StructureSection load='6wkw' size='340' side='right'caption='[[6wkw]]' scene=''> | + | <StructureSection load='6wkw' size='340' side='right'caption='[[6wkw]], [[Resolution|resolution]] 3.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WKW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WKW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WKW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WKW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wkw OCA], [http://pdbe.org/6wkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wkw RCSB], [http://www.ebi.ac.uk/pdbsum/6wkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wkw ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTBP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wkw OCA], [http://pdbe.org/6wkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wkw RCSB], [http://www.ebi.ac.uk/pdbsum/6wkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wkw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CTBP2_HUMAN CTBP2_HUMAN]] Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity). Isoform 2 probably acts as a scaffold for specialized synapses. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | C-terminal binding proteins 1 and 2 (CtBP1 and CtBP2) are transcriptional regulators that activate or repress many genes involved in cellular development, apoptosis, and metastasis. NADH-dependent CtBP activation has been implicated in multiple types of cancer and poor patient prognosis. Central to understanding activation of CtBP in oncogenesis is uncovering how NADH triggers protein assembly, what level of assembly occurs, and if oncogenic activity depends upon such assembly. Here, we present the cryoelectron microscopic structures of two different constructs of CtBP2 corroborating that the native state of CtBP2 in the presence of NADH is tetrameric. The physiological relevance of the observed tetramer was demonstrated in cell culture, showing that CtBP tetramer-destabilizing mutants are defective for cell migration, transcriptional repression of E-cadherin, and activation of TIAM1. Together with our cryoelectron microscopy studies, these results highlight the tetramer as the functional oligomeric form of CtBP2. | ||
| + | |||
| + | Cryo-EM Structure of CtBP2 Confirms Tetrameric Architecture.,Jecrois AM, Dcona MM, Deng X, Bandyopadhyay D, Grossman SR, Schiffer CA, Royer WE Jr Structure. 2020 Nov 27. pii: S0969-2126(20)30420-2. doi:, 10.1016/j.str.2020.11.008. PMID:33264605<ref>PMID:33264605</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wkw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Jecrois | + | [[Category: Jecrois, A M]] |
| + | [[Category: Cancer]] | ||
| + | [[Category: Gene regulation]] | ||
| + | [[Category: Gene repression]] | ||
| + | [[Category: Metabolic sensor]] | ||
| + | [[Category: Transcriptional corepression]] | ||
Revision as of 12:48, 16 December 2020
EM structure of CtBP2 with a minimal dehydrogenase domain of CtBP2
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