1r5p

From Proteopedia

(Difference between revisions)

Revision as of 13:19, 16 December 2020

Crystal Structure Analysis of KaiB from PCC7120

Structural highlights

1r5p is a 2 chain structure with sequence from Anabaena 7120. The January 2008 RCSB PDB Molecule of the Month feature on Circadian Clock Proteins by David S. Goodsell is 10.2210/rcsb_pdb/mom_2008_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1r5q
Gene:	KaiB (Anabaena 7120)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KAIB_NOSS1] Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-non-specific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it decreases the phosphorylation status of KaiC. It has no effect on KaiC by itself, but instead needs the presence of both KaiA and KaiC, suggesting that it acts by antagonizing the interaction between KaiA and KaiC (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The cyanobacterial clock proteins KaiA and KaiB are proposed as regulators of the circadian rhythm in cyanobacteria. Mutations in both proteins have been reported to alter or abolish circadian rhythmicity. Here, we present molecular models of both KaiA and KaiB from the cyanobacteria Anabaena sp PCC7120 deduced by crystal structure analysis, and we discuss how clock-changing or abolishing mutations may cause their resulting circadian phenotype. The overall fold of the KaiA monomer is that of a four-helix bundle. KaiB, on the other hand, adopts an alpha-beta meander motif. Both proteins purify and crystallize as dimers. While the folds of the two proteins are clearly different, their size and some surface features of the physiologically relevant dimers are very similar. Notably, the functionally relevant residues Arg 69 of KaiA and Arg 23 of KaiB align well in space. The apparent structural similarities suggest that KaiA and KaiB may compete for a potential common binding site on KaiC.

Anabaena circadian clock proteins KaiA and KaiB reveal a potential common binding site to their partner KaiC.,Garces RG, Wu N, Gillon W, Pai EF EMBO J. 2004 Apr 21;23(8):1688-98. Epub 2004 Apr 8. PMID:15071498^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Garces RG, Wu N, Gillon W, Pai EF. Anabaena circadian clock proteins KaiA and KaiB reveal a potential common binding site to their partner KaiC. EMBO J. 2004 Apr 21;23(8):1688-98. Epub 2004 Apr 8. PMID:15071498 doi:http://dx.doi.org/10.1038/sj.emboj.7600190

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r5p"

@@ Line 1: / Line 1: @@
 ==Crystal Structure Analysis of KaiB from PCC7120==
-<StructureSection load='1r5p' size='340' side='right' caption='[[1r5p]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='1r5p' size='340' side='right'caption='[[1r5p]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1r5p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. The January 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Circadian Clock Proteins''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_1 10.2210/rcsb_pdb/mom_2008_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R5P FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1r5p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. The January 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Circadian Clock Proteins''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_1 10.2210/rcsb_pdb/mom_2008_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1R5P FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1r5q|1r5q]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1r5q|1r5q]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KaiB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5p OCA], [http://pdbe.org/1r5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r5p RCSB], [http://www.ebi.ac.uk/pdbsum/1r5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5p ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1r5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5p OCA], [http://pdbe.org/1r5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r5p RCSB], [http://www.ebi.ac.uk/pdbsum/1r5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5p ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 29: @@
 </div>
 <div class="pdbe-citations 1r5p" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Circadian clock protein 3D structures|Circadian clock protein 3D structures]]
 == References ==
 <references/>
@@ Line 35: / Line 38: @@
 [[Category: Anabaena 7120]]
 [[Category: Circadian Clock Proteins]]
+[[Category: Large Structures]]
 [[Category: RCSB PDB Molecule of the Month]]
 [[Category: Garces, R G]]

1r5p

From Proteopedia

Revision as of 13:19, 16 December 2020

Crystal Structure Analysis of KaiB from PCC7120

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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