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1rif

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Revision as of 13:27, 16 December 2020

Crystal structure of the UvsW helicase from Bacteriophage T4

Structural highlights

1rif is a 2 chain structure with sequence from Bpt4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	UVSW, DAR (BPT4)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UVSW_BPT4] DNA helicase that catalyzes branch migration and dissociation of RNA-DNA hybrids. Plays a critical role in a specific recombination pathway involving simple reciprocal exchange. UvsW protein is normally the key regulatory factor in the switch from early to late phage DNA replication.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In bacteriophage T4, the WXY system repairs DNA damage by a process that involves homologous recombination. This system comprises three proteins, the RecA-like recombination protein UvsX, a recombination mediator protein UvsY, and a helicase UvsW. Here we report the 2.0 A resolution crystal structure of the N-terminal two domains of the UvsW helicase (UvsWNF; residues 1-282). The structure reveals a typical helicase RecA-like domain linked to a small N-terminal alpha/beta domain that likely binds the nucleic acid substrate. The missing C-terminal portion of UvsW almost certainly corresponds to the second RecA-like domain typically found in monomeric helicases. The putative substrate binding domain is unique within the known helicase structures, and it resembles the novel "double-wing" DNA binding domain from the phage T4 MotA transcription factor that mediates the expression of T4 middle genes. The functional implications of this homology for the role of UvsW in T4 DNA metabolism are discussed.

The crystal structure of the UvsW helicase from bacteriophage T4.,Sickmier EA, Kreuzer KN, White SW Structure. 2004 Apr;12(4):583-92. PMID:15062081^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sickmier EA, Kreuzer KN, White SW. The crystal structure of the UvsW helicase from bacteriophage T4. Structure. 2004 Apr;12(4):583-92. PMID:15062081 doi:10.1016/j.str.2004.02.016

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rif"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the UvsW helicase from Bacteriophage T4==
-<StructureSection load='1rif' size='340' side='right' caption='[[1rif]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='1rif' size='340' side='right'caption='[[1rif]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1rif]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RIF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1rif]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RIF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UVSW, DAR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rif OCA], [http://pdbe.org/1rif PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rif RCSB], [http://www.ebi.ac.uk/pdbsum/1rif PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rif ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rif OCA], [http://pdbe.org/1rif PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rif RCSB], [http://www.ebi.ac.uk/pdbsum/1rif PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rif ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 29: @@
 </div>
 <div class="pdbe-citations 1rif" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Helicase 3D structures|Helicase 3D structures]]
 == References ==
 <references/>
@@ Line 34: / Line 37: @@
 </StructureSection>
 [[Category: Bpt4]]
+[[Category: Large Structures]]
 [[Category: Kreuzer, K N]]
 [[Category: Sickmier, E A]]

1rif

From Proteopedia

Revision as of 13:27, 16 December 2020

Crystal structure of the UvsW helicase from Bacteriophage T4

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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