1rj2

From Proteopedia

(Difference between revisions)

Revision as of 13:27, 16 December 2020

Crystal structure of the DH/PH fragment of Dbs without bound GTPase

Structural highlights

1rj2 is a 4 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1kz7, 1kzg, 1lb1, 1foe
Gene:	MCF2L, OST (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MCF2L_RAT] Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Dbl proteins are guanine nucleotide exchange factors for Rho GTPases, containing adjacent Dbl homology (DH) and pleckstrin homology (PH) domains. This domain architecture is virtually invariant and typically required for full exchange potential. Several structures of DH/PH fragments bound to GTPases implicate the PH domain in nucleotide exchange. To more fully understand the functional linkage between DH and PH domains, we have determined the crystal structure of the DH/PH fragment of Dbs without bound GTPase. This structure is generally similar to previously determined structures of Dbs bound to GTPases albeit with greater apparent mobility between the DH and PH domains. These comparisons suggest that the DH and PH domains of Dbs are spatially primed for binding GTPases and small alterations in intradomain conformations that may be elicited by subtle biological responses, such as altered phosphoinositide levels, are sufficient to enhance exchange by facilitating interactions between the PH domain and GTPases.

Crystal structure of the DH/PH fragment of Dbs without bound GTPase.,Worthylake DK, Rossman KL, Sondek J Structure. 2004 Jun;12(6):1078-86. PMID:15274927^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Worthylake DK, Rossman KL, Sondek J. Crystal structure of the DH/PH fragment of Dbs without bound GTPase. Structure. 2004 Jun;12(6):1078-86. PMID:15274927 doi:10.1016/j.str.2004.03.021

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rj2"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the DH/PH fragment of Dbs without bound GTPase==
-<StructureSection load='1rj2' size='340' side='right' caption='[[1rj2]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+<StructureSection load='1rj2' size='340' side='right'caption='[[1rj2]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1rj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RJ2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1rj2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RJ2 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kz7|1kz7]], [[1kzg|1kzg]], [[1lb1|1lb1]], [[1foe|1foe]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1kz7|1kz7]], [[1kzg|1kzg]], [[1lb1|1lb1]], [[1foe|1foe]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MCF2L, OST ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rj2 OCA], [http://pdbe.org/1rj2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rj2 RCSB], [http://www.ebi.ac.uk/pdbsum/1rj2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rj2 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rj2 OCA], [http://pdbe.org/1rj2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rj2 RCSB], [http://www.ebi.ac.uk/pdbsum/1rj2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rj2 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Lk3 transgenic mice]]
 [[Category: Rossman, K L]]

1rj2

From Proteopedia

Revision as of 13:27, 16 December 2020

Crystal structure of the DH/PH fragment of Dbs without bound GTPase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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