6ysg
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Magnesium chelatase H subunit (ChlH) from Synechocystis sp.PCC6803 to 2.54 A resolution== | ==Magnesium chelatase H subunit (ChlH) from Synechocystis sp.PCC6803 to 2.54 A resolution== | ||
| - | <StructureSection load='6ysg' size='340' side='right'caption='[[6ysg]]' scene=''> | + | <StructureSection load='6ysg' size='340' side='right'caption='[[6ysg]], [[Resolution|resolution]] 2.54Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YSG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YSG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ysg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YSG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YSG FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ysg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ysg OCA], [http://pdbe.org/6ysg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ysg RCSB], [http://www.ebi.ac.uk/pdbsum/6ysg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ysg ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6ys9|6ys9]]</div></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">chlH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ysg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ysg OCA], [http://pdbe.org/6ysg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ysg RCSB], [http://www.ebi.ac.uk/pdbsum/6ysg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ysg ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The insertion of magnesium into protoporphyrin initiates the biosynthesis of chlorophyll, the pigment that underpins photosynthesis. This reaction, catalysed by the magnesium chelatase complex, couples ATP hydrolysis by a ChlID motor complex to chelation within the ChlH subunit. We probed the structure and catalytic function of ChlH using a combination of X-ray crystallography, computational modelling, mutagenesis and enzymology. Two linked domains of ChlH in an initially open conformation of ChlH bind protoporphyrin IX, and the rearrangement of several loops envelops this substrate, forming an active site cavity. This induced fit brings an essential glutamate (E660), proposed to be the key catalytic residue for magnesium insertion, into proximity with the porphyrin. A buried solvent channel adjacent to E660 connects the exterior bulk solvent to the active site, forming a possible conduit for the delivery of magnesium or abstraction of protons. | ||
| + | |||
| + | The active site of magnesium chelatase.,Adams NBP, Bisson C, Brindley AA, Farmer DA, Davison PA, Reid JD, Hunter CN Nat Plants. 2020 Nov 30. pii: 10.1038/s41477-020-00806-9. doi:, 10.1038/s41477-020-00806-9. PMID:33257858<ref>PMID:33257858</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ysg" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Bisson C]] | + | [[Category: Syny3]] |
| - | [[Category: Hunter | + | [[Category: Bisson, C]] |
| + | [[Category: Hunter, C N]] | ||
| + | [[Category: Chlorophyll]] | ||
| + | [[Category: Magnesium chelatase]] | ||
| + | [[Category: Photosynthesis]] | ||
Revision as of 10:59, 24 December 2020
Magnesium chelatase H subunit (ChlH) from Synechocystis sp.PCC6803 to 2.54 A resolution
| |||||||||||
