1t00

From Proteopedia

(Difference between revisions)

Revision as of 11:37, 24 December 2020

The structure of thioredoxin from S. coelicolor

Structural highlights

1t00 is a 1 chain structure with sequence from Strco. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	TRXA, SCO3889, SCH24.11C (STRCO)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[THIO1_STRCO] Component of the thioredoxin-thioredoxin reductase system. Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Stimulates complex formation between sigma factor SigR and its cognate anti-sigma factor RsrA probably by reducing RsrA.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Thioredoxins are ubiquitous proteins that serve as reducing agents and general protein disulfide reductases. In turn, they are reduced by electrons obtained from the NADPH-containing thioredoxin reductase. Thioredoxins have been isolated and characterized from a large number of organisms. The Gram-positive bacterium Streptomyces coelicolor contains three thioredoxins that are involved in unknown biological processes. trxA from S. coelicolor was cloned and expressed in Escherichia coli and the protein purified and crystallized using the hanging-drop method of vapour diffusion. The crystal structure of thioredoxin A has been determined at 1.5 A resolution using a synchrotron-radiation source. The protein reveals a thioredoxin-like fold with a typical CXXC active site. The crystal exhibits the symmetry of space group P2(1)2(1)2, with unit-cell parameters a = 43.6, b = 71.8, c = 33.2 A.

Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor.,Stefankova P, Maderova J, Barak I, Kollarova M, Otwinowski Z Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt, 2):164-8. Epub 2005 Jan 8. PMID:16510983^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kang JG, Paget MS, Seok YJ, Hahn MY, Bae JB, Hahn JS, Kleanthous C, Buttner MJ, Roe JH. RsrA, an anti-sigma factor regulated by redox change. EMBO J. 1999 Aug 2;18(15):4292-8. PMID:10428967 doi:http://dx.doi.org/10.1093/emboj/18.15.4292
↑ Stefankova P, Maderova J, Barak I, Kollarova M, Otwinowski Z. Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt, 2):164-8. Epub 2005 Jan 8. PMID:16510983 doi:10.1107/S1744309104032993

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t00"

@@ Line 1: / Line 1: @@
 ==The structure of thioredoxin from S. coelicolor==
-<StructureSection load='1t00' size='340' side='right' caption='[[1t00]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
+<StructureSection load='1t00' size='340' side='right'caption='[[1t00]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1t00]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T00 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1t00]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T00 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T00 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TRXA, SCO3889, SCH24.11C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t00 OCA], [http://pdbe.org/1t00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t00 RCSB], [http://www.ebi.ac.uk/pdbsum/1t00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t00 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t00 OCA], [http://pdbe.org/1t00 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t00 RCSB], [http://www.ebi.ac.uk/pdbsum/1t00 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t00 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Thioredoxin|Thioredoxin]]
+*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Strco]]
 [[Category: Stefankova, P]]

1t00

From Proteopedia

Revision as of 11:37, 24 December 2020

The structure of thioredoxin from S. coelicolor

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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