1deb
From Proteopedia
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'''CRYSTAL STRUCTURE OF THE N-TERMINAL COILED COIL DOMAIN FROM APC''' | '''CRYSTAL STRUCTURE OF THE N-TERMINAL COILED COIL DOMAIN FROM APC''' | ||
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[[Category: Alber, T.]] | [[Category: Alber, T.]] | ||
[[Category: Day, C L.]] | [[Category: Day, C L.]] | ||
| - | [[Category: | + | [[Category: Apc]] |
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: Tumor suppressor]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:45:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:45, 2 May 2008
CRYSTAL STRUCTURE OF THE N-TERMINAL COILED COIL DOMAIN FROM APC
Overview
Coiled coils serve as dimerization domains for a wide variety of proteins, including the medically important oligomeric tumor suppressor protein, APC. Mutations in the APC gene are associated with an inherited susceptibility to colon cancer and with approximately 75 % of sporadic colorectal tumors. To define the basis for APC pairing and to explore the anatomy of dimeric coiled coils, we determined the 2.4 A resolution X-ray crystal structure of the N-terminal dimerization domain of APC. The peptide APC-55, encompassing the heptad repeats in APC residues 2-55, primarily forms an alpha-helical, coiled-coil dimer with newly observed core packing features. Correlated asymmetric packing of four core residues in distinct, standard rotamers is associated with a small shift in the helix register. At the C terminus, the helices splay apart and interact with a symmetry-related dimer in the crystal to form a short, anti-parallel, four-helix bundle. N-terminal fraying and C-terminal splaying of the helices, as well as the asymmetry and helix register shift describe unprecedented dynamic excursions of coiled coils. The low stability of APC-55 and divergence from the expected coiled-coil fold support the suggestion that the APC dimerization domain may extend beyond the first 55 residues.
About this Structure
1DEB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the amino-terminal coiled-coil domain of the APC tumor suppressor., Day CL, Alber T, J Mol Biol. 2000 Aug 4;301(1):147-56. PMID:10926498 Page seeded by OCA on Fri May 2 13:45:21 2008
