1ded

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[[Image:1ded.gif|left|200px]]
[[Image:1ded.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ded |SIZE=350|CAPTION= <scene name='initialview01'>1ded</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1ded", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACR:ACARBOSE'>ACR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1ded| PDB=1ded | SCENE= }}
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|RELATEDENTRY=[[1d7f|1D7F]], [[1pam|1PAM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ded FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ded OCA], [http://www.ebi.ac.uk/pdbsum/1ded PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ded RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF ALKALOPHILIC ASPARAGINE 233-REPLACED CYCLODEXTRIN GLUCANOTRANSFERASE COMPLEXED WITH AN INHIBITOR, ACARBOSE, AT 2.0 A RESOLUTION'''
'''CRYSTAL STRUCTURE OF ALKALOPHILIC ASPARAGINE 233-REPLACED CYCLODEXTRIN GLUCANOTRANSFERASE COMPLEXED WITH AN INHIBITOR, ACARBOSE, AT 2.0 A RESOLUTION'''
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[[Category: Ishii, N.]]
[[Category: Ishii, N.]]
[[Category: Yamane, K.]]
[[Category: Yamane, K.]]
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[[Category: acarbose]]
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[[Category: Acarbose]]
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[[Category: cgtase]]
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[[Category: Cgtase]]
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[[Category: cyclodextrin glucanotransferase]]
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[[Category: Cyclodextrin glucanotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:45:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:39:58 2008''
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Revision as of 10:45, 2 May 2008

Image:1ded.gif

Template:STRUCTURE 1ded

CRYSTAL STRUCTURE OF ALKALOPHILIC ASPARAGINE 233-REPLACED CYCLODEXTRIN GLUCANOTRANSFERASE COMPLEXED WITH AN INHIBITOR, ACARBOSE, AT 2.0 A RESOLUTION


Overview

The product specificity of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. #1011 is improved to near-uniformity by mutation of histidine-233 to asparagine. Asparagine 233-replaced CGTase (H233N-CGTase) no longer produces alpha-cyclodextrin, while the wild-type CGTase from the same bacterium produces a mixture of predominantly alpha-, beta-, and gamma-cyclodextrins, catalyzing the conversion of starch into cyclic or linear alpha-1,4-linked glucopyranosyl chains. In order to better understand the protein engineering of H233N-CGTase, the crystal structure of the mutant enzyme complexed with a maltotetraose analog, acarbose, was determined at 2.0 A resolution with a final crystallographic R value of 0.163 for all data. Taking a close look at the active site cleft in which the acarbose molecule is bound, the most probable reason for the improved specificity of H233N-CGTase is the removal of interactions needed to form a compact ring like a-cyclodextrin.

About this Structure

1DED is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of alkalophilic asparagine 233-replaced cyclodextrin glucanotransferase complexed with an inhibitor, acarbose, at 2.0 A resolution., Ishii N, Haga K, Yamane K, Harata K, J Biochem. 2000 Mar;127(3):383-91. PMID:10731709 Page seeded by OCA on Fri May 2 13:45:32 2008

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