6lf2
From Proteopedia
(Difference between revisions)
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==SeviL bound to asialo-GM1 saccharide== | ==SeviL bound to asialo-GM1 saccharide== | ||
| - | <StructureSection load='6lf2' size='340' side='right'caption='[[6lf2]]' scene=''> | + | <StructureSection load='6lf2' size='340' side='right'caption='[[6lf2]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LF2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LF2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lf2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LF2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LF2 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lf2 OCA], [http://pdbe.org/6lf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lf2 RCSB], [http://www.ebi.ac.uk/pdbsum/6lf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lf2 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lf2 OCA], [http://pdbe.org/6lf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lf2 RCSB], [http://www.ebi.ac.uk/pdbsum/6lf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lf2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SeviL is a recently isolated lectin found to bind to the linear saccharides of the ganglioside GM1b (Neu5Ac[Formula: see text](2-3)Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc) and its precursor, asialo-GM1 (Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc). The crystal structures of recombinant SeviL have been determined in the presence and absence of ligand. The protein belongs to the [Formula: see text]-trefoil family, but shows only weak sequence similarity to known structures. SeviL forms a dimer in solution, with one binding site per subunit, close to the subunit interface. Molecular details of glycan recognition by SeviL in solution were analysed by ligand- and protein-based NMR techniques as well as ligand binding assays. SeviL shows no interaction with GM1 due to steric hindrance with the sialic acid branch that is absent from GM1b. This unusual specificity makes SeviL of great interest for the detection and control of certain cancer cells, and cells of the immune system, that display asialo-GM1. | ||
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| + | The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata.,Kamata K, Mizutani K, Takahashi K, Marchetti R, Silipo A, Addy C, Park SY, Fujii Y, Fujita H, Konuma T, Ikegami T, Ozeki Y, Tame JRH Sci Rep. 2020 Dec 16;10(1):22102. doi: 10.1038/s41598-020-78926-7. PMID:33328520<ref>PMID:33328520</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lf2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kamata K]] | + | [[Category: Kamata, K]] |
| - | [[Category: Ozeki Y]] | + | [[Category: Ozeki, Y]] |
| - | [[Category: Park S | + | [[Category: Park, S Y]] |
| - | [[Category: Tame | + | [[Category: Tame, J R.H]] |
| + | [[Category: Anti-cancer]] | ||
| + | [[Category: Apoptosis]] | ||
| + | [[Category: Mussel]] | ||
| + | [[Category: Sugar binding protein]] | ||
| + | [[Category: Trefoil]] | ||
Revision as of 06:53, 30 December 2020
SeviL bound to asialo-GM1 saccharide
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