7c7d
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3== | ==Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3== | ||
| - | <StructureSection load='7c7d' size='340' side='right'caption='[[7c7d]]' scene=''> | + | <StructureSection load='7c7d' size='340' side='right'caption='[[7c7d]], [[Resolution|resolution]] 1.16Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C7D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C7D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7c7d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_thermodiastaticus"_bergey_et_al._1923 "actinomyces thermodiastaticus" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C7D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C7D FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c7d OCA], [http://pdbe.org/7c7d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c7d RCSB], [http://www.ebi.ac.uk/pdbsum/7c7d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c7d ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.59 3.2.1.59] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c7d OCA], [http://pdbe.org/7c7d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c7d RCSB], [http://www.ebi.ac.uk/pdbsum/7c7d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c7d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | alpha-1,3-Glucan is a homopolymer composed of D-glucose (Glc) and it is an extracellular polysaccharide found in dental plaque due to Streptococcus species. alpha-1,3-Glucanase from Streptomyces thermodiastaticus strain HF3-3 (Agl-ST) has been identified as a thermostable alpha-1,3-glucanase, which is classified into glycoside hydrolase family 87 (GH87) and specifically hydrolyzes alpha-1,3-glucan with an endo-action. The enzyme has a potential to inhibit the production of dental plaque and to be used for biotechnological applications. Here we show the structure of the catalytic unit of Agl-ST determined at 1.16 A resolution using X-ray crystallography. The catalytic unit is composed of two modules, a beta-sandwich fold module, and a right-handed beta-helix fold module, which resembles other structural characterized GH87 enzymes from Bacillus circulans str. KA-304 and Paenibacillus glycanilyticus str. FH11, with moderate sequence identities between each other (approximately 27% between the catalytic units). However, Agl-ST is smaller in size and more thermally stable than the others. A disulfide bond that anchors the C-terminal coil of the beta-helix fold, which is expected to contribute to thermal stability only exists in the catalytic unit of Agl-ST. | ||
| + | |||
| + | Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3.,Itoh T, Panti N, Hayashi J, Toyotake Y, Matsui D, Yano S, Wakayama M, Hibi T Biochem Biophys Res Commun. 2020 Oct 8. pii: S0006-291X(20)31894-5. doi:, 10.1016/j.bbrc.2020.09.133. PMID:33041007<ref>PMID:33041007</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7c7d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Actinomyces thermodiastaticus bergey et al. 1923]] | ||
| + | [[Category: Hydrolase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Hayashi J]] | + | [[Category: Hayashi, J]] |
| - | [[Category: Hibi T]] | + | [[Category: Hibi, T]] |
| - | [[Category: Itoh T]] | + | [[Category: Itoh, T]] |
| - | [[Category: Panti N]] | + | [[Category: Panti, N]] |
| - | [[Category: Suyotha W]] | + | [[Category: Suyotha, W]] |
| - | [[Category: Toyotake Y]] | + | [[Category: Toyotake, Y]] |
| - | [[Category: Wakayama M]] | + | [[Category: Wakayama, M]] |
| - | [[Category: Yano S]] | + | [[Category: Yano, S]] |
| + | [[Category: Glycosidase]] | ||
Revision as of 07:04, 30 December 2020
Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3
| |||||||||||
