1ufi

From Proteopedia

(Difference between revisions)

Revision as of 07:26, 30 December 2020

Crystal structure of the dimerization domain of human CENP-B

Structural highlights

1ufi is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CENPB (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[CENPB_HUMAN] Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

Publication Abstract from PubMed

The human centromere protein B (CENP-B), a centromeric heterochromatin component, forms a homodimer that specifically binds to a distinct DNA sequence (the CENP-B box), which appears within every other alpha-satellite repeat. Previously, we determined the structure of the human CENP-B DNA-binding domain, CENP-B-(1-129), complexed with the CENP-B box DNA. In the present study, we determined the crystal structure of its dimerization domain (CENP-B-(540-599)), another functional domain of CENP-B, at 1.65-A resolution. CENP-B-(540-599) contains two alpha-helices, which are folded into an antiparallel configuration. The CENP-B-(540-599) dimer formed a symmetrical, antiparallel, four-helix bundle structure with a large hydrophobic patch in which 23 residues of one monomer form van der Waals contacts with the other monomer. In the CENP-B-(540-599) dimer, the N-terminal ends of CENP-B-(540-599) are oriented on opposite sides of the dimer. This CENP-B dimer configuration may be suitable for capturing two distant CENP-B boxes during centromeric heterochromatin formation.

Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution.,Tawaramoto MS, Park SY, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S J Biol Chem. 2003 Dec 19;278(51):51454-61. Epub 2003 Sep 30. PMID:14522975^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tawaramoto MS, Park SY, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S. Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution. J Biol Chem. 2003 Dec 19;278(51):51454-61. Epub 2003 Sep 30. PMID:14522975 doi:http://dx.doi.org/10.1074/jbc.M310388200

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ufi"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the dimerization domain of human CENP-B==
-<StructureSection load='1ufi' size='340' side='right' caption='[[1ufi]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='1ufi' size='340' side='right'caption='[[1ufi]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ufi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UFI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ufi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1UFI FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CENPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufi OCA], [http://pdbe.org/1ufi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ufi RCSB], [http://www.ebi.ac.uk/pdbsum/1ufi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ufi ProSAT], [http://www.topsan.org/Proteins/RSGI/1ufi TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufi OCA], [http://pdbe.org/1ufi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ufi RCSB], [http://www.ebi.ac.uk/pdbsum/1ufi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ufi ProSAT], [http://www.topsan.org/Proteins/RSGI/1ufi TOPSAN]</span></td></tr>
 </table>
 == Function ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Centromere protein|Centromere protein]]
+*[[Centromere protein 3D structure|Centromere protein 3D structure]]
 == References ==
 <references/>
@@ Line 26: / Line 26: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Kurumizaka, H]]
 [[Category: Park, S Y]]

1ufi

From Proteopedia

Revision as of 07:26, 30 December 2020

Crystal structure of the dimerization domain of human CENP-B

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox