1wsu

From Proteopedia

(Difference between revisions)

Revision as of 09:26, 6 January 2021

C-terminal domain of elongation factor selB complexed with SECIS RNA

Structural highlights

1wsu is a 7 chain structure with sequence from Atcc 35608. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	selB (ATCC 35608)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SELB_MOOTH] Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In bacteria, incorporation of selenocysteine, the 21(st) amino acid, into proteins requires elongation factor SelB, which has the unusual property of binding to both transfer RNA (tRNA) and mRNA. SelB binds to an mRNA hairpin formed by the selenocysteine insertion sequence (SECIS) with extremely high specificity, the molecular basis of which has been unknown. We have determined the crystal structure of the mRNA-binding domain of SelB in complex with SECIS RNA at a resolution of 2.3 A. This is the first example of a complex between an RNA and a winged-helix (WH) domain, a motif found in many DNA-binding proteins and recently discovered in RNA-binding proteins. Notably, RNA binding does not induce a major conformational change in the WH motif. The structure reveals a new mode of RNA recognition with a geometry that allows the complex to wrap around the small ribosomal subunit.

Structural basis for mRNA recognition by elongation factor SelB.,Yoshizawa S, Rasubala L, Ose T, Kohda D, Fourmy D, Maenaka K Nat Struct Mol Biol. 2005 Feb;12(2):198-203. Epub 2005 Jan 23. PMID:15665870^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yoshizawa S, Rasubala L, Ose T, Kohda D, Fourmy D, Maenaka K. Structural basis for mRNA recognition by elongation factor SelB. Nat Struct Mol Biol. 2005 Feb;12(2):198-203. Epub 2005 Jan 23. PMID:15665870 doi:nsmb890

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wsu"

@@ Line 1: / Line 1: @@
 ==C-terminal domain of elongation factor selB complexed with SECIS RNA==
-<StructureSection load='1wsu' size='340' side='right' caption='[[1wsu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='1wsu' size='340' side='right'caption='[[1wsu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1wsu]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1wsu]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1WSU FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">selB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1525 ATCC 35608])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsu OCA], [http://pdbe.org/1wsu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wsu RCSB], [http://www.ebi.ac.uk/pdbsum/1wsu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsu ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1wsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsu OCA], [http://pdbe.org/1wsu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wsu RCSB], [http://www.ebi.ac.uk/pdbsum/1wsu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsu ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Elongation factor|Elongation factor]]
+*[[Elongation factor 3D structures|Elongation factor 3D structures]]
 *[[SelB|SelB]]
 == References ==
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Atcc 35608]]
+[[Category: Large Structures]]
 [[Category: Fourmy, D]]
 [[Category: Kohda, D]]

1wsu

From Proteopedia

Revision as of 09:26, 6 January 2021

C-terminal domain of elongation factor selB complexed with SECIS RNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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