1dg6
From Proteopedia
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'''CRYSTAL STRUCTURE OF APO2L/TRAIL''' | '''CRYSTAL STRUCTURE OF APO2L/TRAIL''' | ||
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[[Category: Ultsch, M H.]] | [[Category: Ultsch, M H.]] | ||
[[Category: Vos, A M.de.]] | [[Category: Vos, A M.de.]] | ||
| - | [[Category: | + | [[Category: Cytokine]] |
| - | [[Category: | + | [[Category: Tnf]] |
| - | [[Category: | + | [[Category: Trimer]] |
| - | [[Category: | + | [[Category: Zinc-binding site]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:49:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:49, 2 May 2008
CRYSTAL STRUCTURE OF APO2L/TRAIL
Overview
Apoptosis-inducing ligand 2 (Apo2L, also called TRAIL), a member of the tumor necrosis factor (TNF) family, induces apoptosis in a variety of human tumor cell lines but not in normal cells [Wiley, S. R., Schooley, K., Smolak, P. J., Din, W. S., Huang, C.-P., Nicholl, J. K., Sutherland, G. R., Smith, T. D., Rauch, C., Smith, C. A., and Goodwin, R. G. (1995) Immunity 3, 673-682; Pitti, R. M., Marsters, S. A., Ruppert, S., Donahue, C. J., Moore, A., and Ashkenazi, A. (1996) J. Biol. Chem. 271, 12687-12690]. Here we describe the structure of Apo2L at 1.3 A resolution and use alanine-scanning mutagenesis to map the receptor contact regions. The structure reveals a homotrimeric protein that resembles TNF with receptor-binding epitopes at the interface between monomers. A zinc ion is buried at the trimer interface, coordinated by the single cysteine residue of each monomer. The zinc ion is required for maintaining the native structure and stability and, hence, the biological activity of Apo2L. This is the first example of metal-dependent oligomerization and function of a cytokine.
About this Structure
1DG6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A unique zinc-binding site revealed by a high-resolution X-ray structure of homotrimeric Apo2L/TRAIL., Hymowitz SG, O'Connell MP, Ultsch MH, Hurst A, Totpal K, Ashkenazi A, de Vos AM, Kelley RF, Biochemistry. 2000 Feb 1;39(4):633-40. PMID:10651627 Page seeded by OCA on Fri May 2 13:49:24 2008
