1xsf

From Proteopedia

(Difference between revisions)

Revision as of 10:18, 6 January 2021

Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis

Structural highlights

1xsf is a 1 chain structure with sequence from "bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	Rv1009 ("Bacillus tuberculosis" (Zopf 1883) Klein 1884)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPFB_MYCTO] Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.

The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes.,Cohen-Gonsaud M, Barthe P, Bagneris C, Henderson B, Ward J, Roumestand C, Keep NH Nat Struct Mol Biol. 2005 Mar;12(3):270-3. Epub 2005 Feb 20. PMID:15723078^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cohen-Gonsaud M, Barthe P, Bagneris C, Henderson B, Ward J, Roumestand C, Keep NH. The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes. Nat Struct Mol Biol. 2005 Mar;12(3):270-3. Epub 2005 Feb 20. PMID:15723078 doi:10.1038/nsmb905

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xsf"

@@ Line 1: / Line 1: @@
 ==Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis==
-<StructureSection load='1xsf' size='340' side='right' caption='[[1xsf]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+<StructureSection load='1xsf' size='340' side='right'caption='[[1xsf]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1xsf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XSF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1xsf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XSF FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv1009 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsf OCA], [http://pdbe.org/1xsf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xsf RCSB], [http://www.ebi.ac.uk/pdbsum/1xsf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xsf ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xsf OCA], [http://pdbe.org/1xsf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xsf RCSB], [http://www.ebi.ac.uk/pdbsum/1xsf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xsf ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Barthe, P]]
 [[Category: Cohen-Gonsaud, M]]

1xsf

From Proteopedia

Revision as of 10:18, 6 January 2021

Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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