7anv
From Proteopedia
(Difference between revisions)
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==Mutational and structural analysis of an ancestral D-type dye decolorizing peroxidase== | ==Mutational and structural analysis of an ancestral D-type dye decolorizing peroxidase== | ||
| - | <StructureSection load='7anv' size='340' side='right'caption='[[7anv]]' scene=''> | + | <StructureSection load='7anv' size='340' side='right'caption='[[7anv]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ANV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7ANV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7anv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ANV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7ANV FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7anv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7anv OCA], [http://pdbe.org/7anv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7anv RCSB], [http://www.ebi.ac.uk/pdbsum/7anv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7anv ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7anv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7anv OCA], [http://pdbe.org/7anv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7anv RCSB], [http://www.ebi.ac.uk/pdbsum/7anv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7anv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Dye-decolorizing peroxidases (DyPs) constitute a superfamily of heme-containing peroxidases that are related neither to animal nor to plant peroxidase families. These are divided into four classes (types A, B, C, and D) based on sequence features. The active site of DyPs contains two highly conserved distal ligands, an aspartate and an arginine, the roles of which are still controversial. These ligands have mainly been studied in class A-C bacterial DyPs, largely because no effective recombinant expression systems have been developed for the fungal (D-type) DyPs. In this work, we employ ancestral sequence reconstruction (ASR) to resurrect a D-type DyP ancestor, AncDyPD-b1. Expression of AncDyPD-b1 in Escherichia coli results in large amounts of a heme-containing soluble protein and allows for the first mutagenesis study on the two distal ligands of a fungal DyP. UV-Vis and resonance Raman (RR) spectroscopic analyses, in combination with steady-state kinetics and the crystal structure, reveal fine pH-dependent details about the heme active site structure and show that both the aspartate (D222) and the arginine (R390) are crucial for hydrogen peroxide reduction. Moreover, the data indicate that these two residues play important but mechanistically different roles on the intraprotein long-range electron transfer process. DATABASE: Structural data are available in the PDB database under the accession number 7ANV. | ||
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| + | Mutational and structural analysis of an ancestral fungal dye-decolorizing peroxidase.,Zitare UA, Habib MH, Rozeboom H, Mascotti ML, Todorovic S, Fraaije MW FEBS J. 2020 Dec 23. doi: 10.1111/febs.15687. PMID:33369202<ref>PMID:33369202</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7anv" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fraaije | + | [[Category: Fraaije, M W]] |
| - | [[Category: Rozeboom | + | [[Category: Rozeboom, H J]] |
| + | [[Category: D-type dyp]] | ||
| + | [[Category: Dye decolorizing peroxidase]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 06:03, 20 January 2021
Mutational and structural analysis of an ancestral D-type dye decolorizing peroxidase
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