7c2s
From Proteopedia
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==Helical reconstruction of Dengue virus serotype 3 complexed with Fab C10== | ==Helical reconstruction of Dengue virus serotype 3 complexed with Fab C10== | ||
| - | <StructureSection load='7c2s' size='340' side='right'caption='[[7c2s]]' scene=''> | + | <StructureSection load='7c2s' size='340' side='right'caption='[[7c2s]], [[Resolution|resolution]] 10.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C2S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C2S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7c2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C2S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C2S FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c2s OCA], [http://pdbe.org/7c2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c2s RCSB], [http://www.ebi.ac.uk/pdbsum/7c2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c2s ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c2s OCA], [http://pdbe.org/7c2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c2s RCSB], [http://www.ebi.ac.uk/pdbsum/7c2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c2s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Previous flavivirus (dengue and Zika viruses) studies showed largely spherical particles either with smooth or bumpy surfaces. Here, we demonstrate flavivirus particles have high structural plasticity by the induction of a non-spherical morphology at elevated temperatures: the club-shaped particle (clubSP), which contains a cylindrical tail and a disc-like head. Complex formation of DENV and ZIKV with Fab C10 stabilize the viruses allowing cryoEM structural determination to ~10 A resolution. The caterpillar-shaped (catSP) Fab C10:ZIKV complex shows Fabs locking the E protein raft structure containing three E dimers. However, compared to the original spherical structure, the rafts have rotated relative to each other. The helical tail structure of Fab C10:DENV3 clubSP showed although the Fab locked an E protein dimer, the dimers have shifted laterally. Morphological diversity, including clubSP and the previously identified bumpy and smooth-surfaced spherical particles, may help flavivirus survival and immune evasion. | ||
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| + | High flavivirus structural plasticity demonstrated by a non-spherical morphological variant.,Morrone SR, Chew VSY, Lim XN, Ng TS, Kostyuchenko VA, Zhang S, Wirawan M, Chew PL, Lee J, Tan JL, Wang J, Tan TY, Shi J, Screaton G, Morais MC, Lok SM Nat Commun. 2020 Jun 19;11(1):3112. doi: 10.1038/s41467-020-16925-y. PMID:32561757<ref>PMID:32561757</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7c2s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Dengue virus 3]] | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chew | + | [[Category: Chew, S V]] |
| - | [[Category: Kostyuchenko | + | [[Category: Kostyuchenko, V A]] |
| - | [[Category: Lim | + | [[Category: Lim, X N]] |
| - | [[Category: Lok | + | [[Category: Lok, S M]] |
| - | [[Category: Morrone S]] | + | [[Category: Morrone, S]] |
| - | [[Category: Ng | + | [[Category: Ng, T S]] |
| - | [[Category: Zhang S]] | + | [[Category: Zhang, S]] |
| + | [[Category: Antibody]] | ||
| + | [[Category: Neutralization]] | ||
| + | [[Category: Virus]] | ||
Revision as of 06:04, 20 January 2021
Helical reconstruction of Dengue virus serotype 3 complexed with Fab C10
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Categories: Dengue virus 3 | Human | Large Structures | Chew, S V | Kostyuchenko, V A | Lim, X N | Lok, S M | Morrone, S | Ng, T S | Zhang, S | Antibody | Neutralization | Virus
