7dag
From Proteopedia
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==Vibrio cholera aldehyde-alcohol dehrogenase== | ==Vibrio cholera aldehyde-alcohol dehrogenase== | ||
| - | <StructureSection load='7dag' size='340' side='right'caption='[[7dag]]' scene=''> | + | <StructureSection load='7dag' size='340' side='right'caption='[[7dag]], [[Resolution|resolution]] 4.37Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DAG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7DAG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7dag]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DAG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7DAG FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7dag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dag OCA], [http://pdbe.org/7dag PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7dag RCSB], [http://www.ebi.ac.uk/pdbsum/7dag PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7dag ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">adhE_1, adhC, adhE, adhE_2, BC353_06450, C9J66_00760, D6U80_02570, ERS013138_00532, ERS013165_00179, ERS013186_01240, ERS013198_03108, ERS013199_01145, ERS013201_00485, ERS013202_01518, ERS013206_03377, FLM02_10510, FXF03_12520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7dag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dag OCA], [http://pdbe.org/7dag PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7dag RCSB], [http://www.ebi.ac.uk/pdbsum/7dag PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7dag ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aldehyde-alcohol dehydrogenase (AdhE) is a metabolic enzyme and virulence factor in bacteria. E. coli AdhE (eAdhE) multimerizes into spirosomes that are essential for enzymatic activity. However, it is unknown whether AdhE structure is conserved in divergent bacteria. Here, we present the cryo-EM structure of AdhE (vAdhE) from Vibrio cholerae to 4.31 A resolution. Overall, vAdhE spirosomes are similar to eAdhE with conserved subunit arrangement. However, divergences in key oligomerization residues cause vAdhE to form labile spirosomes with lower enzymatic activity. Mutating the vAdhE oligomerization interface to mimic eAdhE increases spirosome stability and enzymatic activity to levels comparable to eAdhE. These results support the generality of AdhE spirosome structures, and provide a structural basis to target vAdhE to attenuate bacterial virulence. | ||
| + | |||
| + | Cryo-EM structure of Vibrio cholerae aldehyde-alcohol dehydrogenase spirosomes.,Cho S, Kim G, Song JJ, Cho C Biochem Biophys Res Commun. 2021 Jan 15;536:38-44. doi:, 10.1016/j.bbrc.2020.12.040. Epub 2020 Dec 23. PMID:33360541<ref>PMID:33360541</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7dag" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillo virgola del koch trevisan 1884]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cho C]] | + | [[Category: Cho, C]] |
| - | [[Category: Cho S]] | + | [[Category: Cho, S]] |
| - | [[Category: Kim G]] | + | [[Category: Kim, G]] |
| - | [[Category: Song J]] | + | [[Category: Song, J]] |
| + | [[Category: Alcohol]] | ||
| + | [[Category: Aldehyde]] | ||
| + | [[Category: Enzyme]] | ||
| + | [[Category: Fermentation]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 06:05, 20 January 2021
Vibrio cholera aldehyde-alcohol dehrogenase
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