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1y9j

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Revision as of 08:42, 20 January 2021

Solution structure of the rat Sly1 N-terminal domain

Structural highlights

1y9j is a 1 chain structure with sequence from Buffalo rat. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	Scfd1, Ra410, Sly1, Stxbp1l2 (Buffalo rat)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCFD1_RAT] Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sec1/Mun18-like (SM) proteins and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) play central roles in intracellular membrane fusion. Diverse modes of interaction between SM proteins and SNAREs from the syntaxin family have been described. However, the observation that the N-terminal domains of Sly1 and Vps45, the SM proteins involved in traffic at the endoplasmic reticulum, the Golgi, the trans-Golgi network and the endosomes, bind to similar N-terminal sequences of their cognate syntaxins suggested a unifying theme for SM protein/SNARE interactions in most internal membrane compartments. To further understand this mechanism of SM protein/SNARE coupling, we have elucidated the structure in solution of the isolated N-terminal domain of rat Sly1 (rSly1N) and analyzed its complex with an N-terminal peptide of rat syntaxin 5 by NMR spectroscopy. Comparison with the crystal structure of a complex between Sly1p and Sed5p, their yeast homologues, shows that syntaxin 5 binding requires a striking conformational change involving a two-residue shift in the register of the C-terminal beta-strand of rSly1N. This conformational change is likely to induce a significant alteration in the overall shape of full-length rSly1 and may be critical for its function. Sequence analyses indicate that this conformational change is conserved in the Sly1 family but not in other SM proteins, and that the four families represented by the four SM proteins found in yeast (Sec1p, Sly1p, Vps45p and Vps33p) diverged early in evolution. These results suggest that there are marked distinctions between the mechanisms of action of each of the four families of SM proteins, which may have arisen from different regulatory requirements of traffic in their corresponding membrane compartments.

Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5.,Arac D, Dulubova I, Pei J, Huryeva I, Grishin NV, Rizo J J Mol Biol. 2005 Feb 18;346(2):589-601. Epub 2004 Dec 24. PMID:15670607^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dascher C, Balch WE. Mammalian Sly1 regulates syntaxin 5 function in endoplasmic reticulum to Golgi transport. J Biol Chem. 1996 Jul 5;271(27):15866-9. PMID:8663406
↑ Matsuo N, Ogawa S, Takagi T, Wanaka A, Mori T, Matsuyama T, Pinsky DJ, Stern DM, Tohyama M. Cloning of a putative vesicle transport-related protein, RA410, from cultured rat astrocytes and its expression in ischemic rat brain. J Biol Chem. 1997 Jun 27;272(26):16438-44. PMID:9195952
↑ Laufman O, Kedan A, Hong W, Lev S. Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing. EMBO J. 2009 Jul 22;28(14):2006-17. Epub 2009 Jun 18. PMID:19536132 doi:emboj2009168
↑ Arac D, Dulubova I, Pei J, Huryeva I, Grishin NV, Rizo J. Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5. J Mol Biol. 2005 Feb 18;346(2):589-601. Epub 2004 Dec 24. PMID:15670607 doi:10.1016/j.jmb.2004.12.004

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y9j"

@@ Line 1: / Line 1: @@
 ==Solution structure of the rat Sly1 N-terminal domain==
-<StructureSection load='1y9j' size='340' side='right' caption='[[1y9j]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1y9j' size='340' side='right'caption='[[1y9j]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1y9j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y9J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1y9j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y9J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Y9J FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Scfd1, Ra410, Sly1, Stxbp1l2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9j OCA], [http://pdbe.org/1y9j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y9j RCSB], [http://www.ebi.ac.uk/pdbsum/1y9j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9j ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1y9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y9j OCA], [http://pdbe.org/1y9j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y9j RCSB], [http://www.ebi.ac.uk/pdbsum/1y9j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y9j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Buffalo rat]]
+[[Category: Large Structures]]
 [[Category: Arac, D]]
 [[Category: Dulubova, I]]

1y9j

From Proteopedia

Revision as of 08:42, 20 January 2021

Solution structure of the rat Sly1 N-terminal domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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