1z4o

From Proteopedia

(Difference between revisions)

Revision as of 08:57, 20 January 2021

Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate

Structural highlights

1z4o is a 2 chain structure with sequence from "bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1z4n
Gene:	pgmB ("Bacterium lactis" Lister 1873)
Activity:	Beta-phosphoglucomutase, with EC number 5.4.2.6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PGMB_LACLA] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.

Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase.,Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Qian N, Stanley GA, Bunte A, Radstrom P. Product formation and phosphoglucomutase activities in Lactococcus lactis: cloning and characterization of a novel phosphoglucomutase gene. Microbiology. 1997 Mar;143 ( Pt 3):855-65. PMID:9084169
↑ Lahiri SD, Zhang G, Dai J, Dunaway-Mariano D, Allen KN. Analysis of the substrate specificity loop of the HAD superfamily cap domain. Biochemistry. 2004 Mar 16;43(10):2812-20. PMID:15005616 doi:10.1021/bi0356810
↑ Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN. Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149 doi:http://dx.doi.org/10.1021/ja0509073

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z4o"

@@ Line 1: / Line 1: @@
 ==Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate==
-<StructureSection load='1z4o' size='340' side='right' caption='[[1z4o]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='1z4o' size='340' side='right'caption='[[1z4o]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1z4o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z4O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z4O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1z4o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z4O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Z4O FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GL1:1-O-PHOSPHONO-ALPHA-D-GALACTOPYRANOSE'>GL1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GL1:1-O-PHOSPHONO-ALPHA-D-GALACTOPYRANOSE'>GL1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1z4n|1z4n]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1z4n|1z4n]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pgmB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z4o OCA], [http://pdbe.org/1z4o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z4o RCSB], [http://www.ebi.ac.uk/pdbsum/1z4o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z4o ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1z4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z4o OCA], [http://pdbe.org/1z4o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z4o RCSB], [http://www.ebi.ac.uk/pdbsum/1z4o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z4o ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 31: / Line 31: @@
 </div>
 <div class="pdbe-citations 1z4o" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Beta-phosphoglucomutase 3D structures|Beta-phosphoglucomutase 3D structures]]
 == References ==
 <references/>
@@ Line 37: / Line 40: @@
 [[Category: Bacterium lactis lister 1873]]
 [[Category: Beta-phosphoglucomutase]]
+[[Category: Large Structures]]
 [[Category: Allen, K N]]
 [[Category: Dai, J]]

1z4o

From Proteopedia

Revision as of 08:57, 20 January 2021

Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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