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2avw

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Revision as of 08:38, 27 January 2021

Crystal structure of monoclinic form of streptococcus Mac-1

Structural highlights

2avw is a 6 chain structure with sequence from Strpv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	mac (STRPV)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2 that mediate host immune evasion by targeting both IgG and Fc receptors. Here, we report the crystal structures of Mac-1 and its catalytically inactive C94A mutant in two different crystal forms. Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold. Alanine mutations at the active site confirmed the critical residues involved in a papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both crystal forms and displays the unique dimer interface among papain superfamily members. Mutations at the dimer interface resulted in a significant reduction in IgG binding and catalysis, suggesting that the dimer contributes to both IgG specificity and enzyme cooperativity. A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2.

Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG.,Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD Structure. 2006 Feb;14(2):225-35. PMID:16472742^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD. Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG. Structure. 2006 Feb;14(2):225-35. PMID:16472742 doi:10.1016/j.str.2005.10.012

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2avw"

@@ Line 1: / Line 1: @@
 ==Crystal structure of monoclinic form of streptococcus Mac-1==
-<StructureSection load='2avw' size='340' side='right' caption='[[2avw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='2avw' size='340' side='right'caption='[[2avw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2avw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpv Strpv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AVW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2avw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Strpv Strpv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AVW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mac ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=293653 STRPV])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mac ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=293653 STRPV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2avw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avw OCA], [http://pdbe.org/2avw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2avw RCSB], [http://www.ebi.ac.uk/pdbsum/2avw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2avw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2avw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avw OCA], [https://pdbe.org/2avw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2avw RCSB], [https://www.ebi.ac.uk/pdbsum/2avw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2avw ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Strpv]]
 [[Category: Agniswamy, J]]

2avw

From Proteopedia

Revision as of 08:38, 27 January 2021

Crystal structure of monoclinic form of streptococcus Mac-1

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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