2nuo

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Revision as of 12:47, 8 November 2007

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spinqualitylabelsall models 2nuo, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of a complex of griffithsin with glucose

Overview

Crystal structures of complexes of an antiviral lectin griffithsin (GRFT), with glucose and N-acetylglucosamine were solved and refined at high, resolution. In both complexes, all six monosaccharide-binding sites of, GRFT were occupied and the mode of binding was similar to that of mannose., In our previous attempts to obtain a complex with N-acetylglucosamine by, soaking, only a single site was occupied; thus, cocrystallization was, clearly superior despite lower concentration of the ligand. Isothermal, titration calorimetric experiments with N-acetylglucosamine, glucose, and, mannose provided enthalpic evidence of distinct binding differences, between the three monosaccharides. A comparison of the mode of binding of, different monosaccharides is discussed in the context of the antiviral, activity of GRFT, based on specific binding to high-mannose-containing, complex carbohydrates found on viral envelopes.

About this Structure

2NUO is a Single protein structure of sequence from Griffithsia with GLC, SO4 and EDO as ligands. Full crystallographic information is available from OCA.

Reference

Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736

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