2b5a

From Proteopedia

(Difference between revisions)

Revision as of 11:35, 3 February 2021

C.BclI, Control Element of the BclI Restriction-Modification System

Structural highlights

2b5a is a 4 chain structure with sequence from 'bacillus caldolyticus'. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	bclIC ('Bacillus caldolyticus')
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Protection from DNA invasion is afforded by restriction-modification systems in many bacteria. The efficiency of protection depends crucially on the relative expression levels of restriction versus methytransferase genes. This regulation is provided by a controller protein, named C protein. Studies of the Bcll system in E. coli suggest that C.Bcll functions as a negative regulator for M.Bcll expression, implying that it plays a role in defense against foreign DNA during virus infection. C.Bcll binds (Kd = 14.3 nM) to a 2-fold symmetric C box DNA sequence that overlaps with the putative -35 promoter region upstream of the bcllM and bcllC genes. The C.Bcll fold comprises five alpha helices: two helices form a helix-turn-helix motif, and the remaining three helices form the extensive dimer interface. The C.Bcll-DNA model proposed suggests that DNA bending might play an important role in gene regulation, and that Glu27 and Asp31 in C.Bcll might function critically in the regulation.

Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site.,Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK Structure. 2005 Dec;13(12):1837-47. PMID:16338412^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK. Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site. Structure. 2005 Dec;13(12):1837-47. PMID:16338412 doi:10.1016/j.str.2005.08.017

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2b5a"

@@ Line 1: / Line 1: @@
 ==C.BclI, Control Element of the BclI Restriction-Modification System==
-<StructureSection load='2b5a' size='340' side='right' caption='[[2b5a]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
+<StructureSection load='2b5a' size='340' side='right'caption='[[2b5a]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2b5a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'bacillus_caldolyticus' 'bacillus caldolyticus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B5A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2b5a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/'bacillus_caldolyticus' 'bacillus caldolyticus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5A FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bclIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1394 'Bacillus caldolyticus'])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bclIC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1394 'Bacillus caldolyticus'])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5a OCA], [http://pdbe.org/2b5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b5a RCSB], [http://www.ebi.ac.uk/pdbsum/2b5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5a ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5a OCA], [https://pdbe.org/2b5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5a RCSB], [https://www.ebi.ac.uk/pdbsum/2b5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5a ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 12: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5a_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 32: / Line 32: @@
 </StructureSection>
 [[Category: Bacillus caldolyticus]]
+[[Category: Large Structures]]
 [[Category: Balendiran, G K]]
 [[Category: Chan, S H]]

2b5a

From Proteopedia

Revision as of 11:35, 3 February 2021

C.BclI, Control Element of the BclI Restriction-Modification System

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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