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| | ==Solution structure of the PABC domain from Triticum aevestium poly(A)-binding protein== | | ==Solution structure of the PABC domain from Triticum aevestium poly(A)-binding protein== |
| - | <StructureSection load='2dyd' size='340' side='right' caption='[[2dyd]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='2dyd' size='340' side='right'caption='[[2dyd]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2dyd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DYD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2dyd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DYD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PABP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Canadian hard winter wheat])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PABP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Canadian hard winter wheat])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dyd OCA], [http://pdbe.org/2dyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dyd RCSB], [http://www.ebi.ac.uk/pdbsum/2dyd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2dyd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dyd OCA], [https://pdbe.org/2dyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dyd RCSB], [https://www.ebi.ac.uk/pdbsum/2dyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dyd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/P93616_WHEAT P93616_WHEAT]] Binds the poly(A) tail of mRNA.[RuleBase:RU362004] | + | [[https://www.uniprot.org/uniprot/P93616_WHEAT P93616_WHEAT]] Binds the poly(A) tail of mRNA.[RuleBase:RU362004] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Canadian hard winter wheat]] | | [[Category: Canadian hard winter wheat]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Siddiqui, N]] | | [[Category: Siddiqui, N]] |
| | [[Category: Alpha helical protein]] | | [[Category: Alpha helical protein]] |
| | [[Category: Rna binding protein]] | | [[Category: Rna binding protein]] |
| Structural highlights
Function
[P93616_WHEAT] Binds the poly(A) tail of mRNA.[RuleBase:RU362004]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In animals, the PABC domain from poly (A)-binding protein recruits proteins containing a specific interacting motif (PAM-2) to the mRNP complex. These proteins include Paip1, Paip2, and eukaryotic release factor 3 (eRF3), all of which regulate PABP function in translation. The following reports the solution structure of PABC from Triticum avestium (wheat) poly (A)-binding protein determined by NMR spectroscopy. Wheat PABC (wPABC) is an alpha-helical protein domain, which displays a fold highly similar to the human PABC domain and contains a PAM-2 peptide binding site. Through a bioinformatics search, several plant proteins containing a PAM-2 site were identified including the early response to dehydration protein (ERD-15), which was previously shown to regulate PABP-dependent translation. The plant PAM-2 proteins contain a variety of conserved sequences including a PABP-interacting 1 motif (PAM-1), RNA binding domains, an SMR endonuclease domain, and a poly (A)-nuclease regulatory domain, all of which suggest a function in either translation or mRNA metabolism. The proteins identified are well conserved throughout plant species but have no sequence homologues in metazoans. We show that wPABC binds to the plant PAM-2 motif with high affinity through a conserved mechanism. Overall, our results suggest that plant species have evolved a distinct regulatory mechanism involving novel PABP binding partners.
Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants.,Siddiqui N, Osborne MJ, Gallie DR, Gehring K Biochemistry. 2007 Apr 10;46(14):4221-31. Epub 2007 Mar 15. PMID:17358048[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Siddiqui N, Osborne MJ, Gallie DR, Gehring K. Solution structure of the PABC domain from wheat poly (A)-binding protein: an insight into RNA metabolic and translational control in plants. Biochemistry. 2007 Apr 10;46(14):4221-31. Epub 2007 Mar 15. PMID:17358048 doi:10.1021/bi061986d
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