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2f0c

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Revision as of 12:36, 10 February 2021

Structure of the Receptor Binding Protein (ORF49, bbp) from lactophage tp901-1

Structural highlights

2f0c is a 3 chain structure with sequence from Bacteriophage tp901-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2bsd, 1zru, 2bse
Gene:	ORF49/bbp (Bacteriophage TP901-1)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lactococcus lactis is a gram-positive bacterium widely used by the dairy industry. Several industrial L. lactis strains are sensitive to various distinct bacteriophages. Most of them belong to the Siphoviridae family and comprise several species, among which the 936 and P335 are prominent. Members of these two phage species recognize their hosts through the interaction of their receptor-binding protein (RBP) with external cell wall saccharidices of the host, the "receptors." We report here the 1.65 A resolution crystal structure of the RBP from phage TP901-1, a member of the P335 species. This RBP of 163 amino acids is a homotrimer comprising three domains: a helical N terminus, an interlaced beta-prism, and a beta-barrel, the head domain (residues 64-163), which binds a glycerol molecule. Fluorescence quenching experiments indicated that the RBP exhibits high affinity for glycerol, muramyl-dipeptide, and other saccharides in solution. The structural comparison of this RBP with that of lactococcal phage p2 RBP, a member of the 936 species (Spinelli, S., Desmyter, A., Verrips, C. T., de Haard, J. W., Moineau, S., and Cambillau, C. (2006) Nat. Struct. Mol. Biol. 13, 85-89) suggests a large extent of modularity in RBPs of lactococcal phages.

Modular structure of the receptor binding proteins of Lactococcus lactis phages. The RBP structure of the temperate phage TP901-1.,Spinelli S, Campanacci V, Blangy S, Moineau S, Tegoni M, Cambillau C J Biol Chem. 2006 May 19;281(20):14256-62. Epub 2006 Mar 20. PMID:16549427^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Spinelli S, Campanacci V, Blangy S, Moineau S, Tegoni M, Cambillau C. Modular structure of the receptor binding proteins of Lactococcus lactis phages. The RBP structure of the temperate phage TP901-1. J Biol Chem. 2006 May 19;281(20):14256-62. Epub 2006 Mar 20. PMID:16549427 doi:10.1074/jbc.M600666200

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f0c"

@@ Line 1: / Line 1: @@
 ==Structure of the Receptor Binding Protein (ORF49, bbp) from lactophage tp901-1==
-<StructureSection load='2f0c' size='340' side='right' caption='[[2f0c]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='2f0c' size='340' side='right'caption='[[2f0c]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2f0c]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_tp901-1 Bacteriophage tp901-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F0C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2f0c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_tp901-1 Bacteriophage tp901-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F0C FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bsd|2bsd]], [[1zru|1zru]], [[2bse|2bse]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bsd|2bsd]], [[1zru|1zru]], [[2bse|2bse]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF49/bbp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35345 Bacteriophage TP901-1])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF49/bbp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35345 Bacteriophage TP901-1])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f0c OCA], [http://pdbe.org/2f0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f0c RCSB], [http://www.ebi.ac.uk/pdbsum/2f0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f0c ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f0c OCA], [https://pdbe.org/2f0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f0c RCSB], [https://www.ebi.ac.uk/pdbsum/2f0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f0c ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 33: / Line 33: @@
 </StructureSection>
 [[Category: Bacteriophage tp901-1]]
+[[Category: Large Structures]]
 [[Category: Cambillau, C]]
 [[Category: Spinelli, S]]

2f0c

From Proteopedia

Revision as of 12:36, 10 February 2021

Structure of the Receptor Binding Protein (ORF49, bbp) from lactophage tp901-1

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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