Mycobacterium Tuberculosis Transpeptidase Domain
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Mtb Transpeptidase Domain in Complex with Penicillin== | ==Mtb Transpeptidase Domain in Complex with Penicillin== | ||
| - | <StructureSection load='5cxw' size='340' side='right' caption='M. tuberculosis penicillin-binding protein | + | <StructureSection load='5cxw' size='340' side='right' caption='M. tuberculosis penicillin-binding protein transpeptidase domain complex with penicillin V, HEPES and PEG (PDB code [[5cxw]]).' scene=''> |
Tuberculosis (TB) is one of the major human respiratory diseases which is caused by ''Mycobacterium Tuberculosis''. The infection of M. tuberculosis causes a large area of necrosis of pulmonary tissue, which results in significant breathing difficulties. TB is also known to be a major causing agent for chronic obstructive pulmonary disease (COPD). Nowadays, TB proposes a significant problem in antibiotic resistance, traditional antibiotics gradually become less and less effective towards the antibiotic-resistant strain of M. tuberculosis. In order to develop a novel antibiotic, further research has led us into the mechanism of how known antibiotic acts on M. tuberculosis. A new therapeutic approach is taken by investigating the penicillium binding site PonA1. | Tuberculosis (TB) is one of the major human respiratory diseases which is caused by ''Mycobacterium Tuberculosis''. The infection of M. tuberculosis causes a large area of necrosis of pulmonary tissue, which results in significant breathing difficulties. TB is also known to be a major causing agent for chronic obstructive pulmonary disease (COPD). Nowadays, TB proposes a significant problem in antibiotic resistance, traditional antibiotics gradually become less and less effective towards the antibiotic-resistant strain of M. tuberculosis. In order to develop a novel antibiotic, further research has led us into the mechanism of how known antibiotic acts on M. tuberculosis. A new therapeutic approach is taken by investigating the penicillium binding site PonA1. | ||
== Function == | == Function == | ||
| - | Two types of [[Penicillin-binding protein]]s (PBPs) can be found in M. tuberculosis, which is PonA1 and PonA2. PonA1 is responsible for the integrity of the poly peptidoglycans, which also regulates the cell wall in the growth of the cell cycle. From research suggest that the function of PBPs PonA2 possess the ability to sense cell cycle and responsible for protein-carbohydrates interactions. | + | Two types of [[Penicillin-binding protein]]s (PBPs) can be found in ''M. tuberculosis'', which is PonA1 and PonA2. PonA1 is responsible for the integrity of the poly peptidoglycans, which also regulates the cell wall in the growth of the cell cycle. From research suggest that the function of PBPs PonA2 possess the ability to sense cell cycle and responsible for protein-carbohydrates interactions. |
When the ß-lactams bind to the PBPs, it induces the degradation of the cell wall by inhibits the activity of transpeptidase. | When the ß-lactams bind to the PBPs, it induces the degradation of the cell wall by inhibits the activity of transpeptidase. | ||
Current revision
Mtb Transpeptidase Domain in Complex with Penicillin
| |||||||||||
References
Filippova, E. V., Kieser, K. J., Luan, C. H., Wawrzak, Z., Kiryukhina, O., Rubin, E. J., & Anderson, W. F. (2016). Crystal structures of the transpeptidase domain of the Mycobacterium tuberculosis penicillin‐binding protein PonA1 reveal potential mechanisms of antibiotic resistance. The FEBS journal, 283(12), 2206-2218.
Maria-Magdalena Patru, Martin S. Pavelka, Jr. A Role for the Class A Penicillin-Binding Protein PonA2 in the Survival of Mycobacterium smegmatis under Conditions of Nonreplication, J Bacteriol. 2010 Jun; 192(12): 3043–3054. Published online 2010 Apr 16. doi: 10.1128/JB.00025-10
