1dqf
From Proteopedia
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'''CRYSTAL STRUCTURE OF HELIX II OF THE X. LAEVIS SOMATIC 5S RRNA WITH A CYTOSINE BULGE IN TWO CONFORMATIONS''' | '''CRYSTAL STRUCTURE OF HELIX II OF THE X. LAEVIS SOMATIC 5S RRNA WITH A CYTOSINE BULGE IN TWO CONFORMATIONS''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQF OCA]. | |
==Reference== | ==Reference== | ||
Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge., Xiong Y, Sundaralingam M, RNA. 2000 Sep;6(9):1316-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10999608 10999608] | Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge., Xiong Y, Sundaralingam M, RNA. 2000 Sep;6(9):1316-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10999608 10999608] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Sundaralingam, M.]] | [[Category: Sundaralingam, M.]] | ||
[[Category: Xiong, Y.]] | [[Category: Xiong, Y.]] | ||
| - | [[Category: | + | [[Category: Cytosine bulge]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:09:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:09, 2 May 2008
CRYSTAL STRUCTURE OF HELIX II OF THE X. LAEVIS SOMATIC 5S RRNA WITH A CYTOSINE BULGE IN TWO CONFORMATIONS
Overview
The crystal structure of r(GCCACCCUG).r(CAGGGUCGGC), helix II of the Xenopus laevis 5S rRNA with a cytosine bulge (underlined), has been determined in two forms at 2.2 A (Form I, space group P4(2)2(1)2, a = b = 57.15 A and c = 43.54 A) and 1.7 A (Form II, space group P4(3)2(1)2, a = b = 32.78 A and c = 102.5 A). The helical regions of the nonamers are found in the standard A-RNA conformations and the two forms have an RMS deviation of 0.75 A. However, the cytosine bulge adopts two significantly different conformations with an RMS deviation of 3.9 A. In Form I, the cytosine bulge forms an intermolecular C+*G.C triple in the major groove of a symmetry-related duplex with intermolecular hydrogen bonds between N4C and O6G, and between protonated N3+C and N7G. In contrast, a minor groove C*G.C triple is formed in Form II with intermolecular hydrogen bonds between O2C and N2G, and between N3C and N3G with a water bridge. A partial major groove opening was observed in Form I structure at the bulge site. Two Ca2+ ions were found in Form I helix whereas there were none in Form II. The structural comparison of these two forms indicates that bulged residues can adopt a variety of conformations with little perturbation to the global helix structure. This suggests that bulged residues could function as flexible latches in bridging double helical motifs and facilitate the folding of large RNA molecules.
About this Structure
Full crystallographic information is available from OCA.
Reference
Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge., Xiong Y, Sundaralingam M, RNA. 2000 Sep;6(9):1316-24. PMID:10999608 Page seeded by OCA on Fri May 2 14:09:00 2008
