398d
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(New page: 200px<br /> <applet load="398d" size="450" color="white" frame="true" align="right" spinBox="true" caption="398d, resolution 1.940Å" /> '''3'-DNA-RNA-5' JUNC...)
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Revision as of 12:51, 8 November 2007
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3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION
Overview
During initiation of minus-strand synthesis by HIV-1 reverse, transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of, tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand, specifically, opposite the newly synthesized DNA strand. We have, determined the crystal structure at 1.9 A resolution of an eight-base pair, hybrid duplex representing the junction to identify global or local, structural perturbations which may be recognized by HIV-RT RNase H. The, junction octamer is in a global A-type conformation throughout. A base, pair step with distinct stacking geometry and variable backbone, conformation is located next to the main endonucleolytic cleavage site., This base pair step may serve as a recognition site for HIV-RT RNase H.
About this Structure
398D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication., Mueller U, Maier G, Mochi Onori A, Cellai L, Heumann H, Heinemann U, Biochemistry. 1998 Sep 1;37(35):12005-11. PMID:9724510
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