1w9d
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ==== | + | ==S. alba myrosinase in complex with S-ethyl phenylacetothiohydroximate- O-sulfate== |
| - | <StructureSection load='1w9d' size='340' side='right'caption='[[1w9d]]' scene=''> | + | <StructureSection load='1w9d' size='340' side='right'caption='[[1w9d]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1w9d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W9D FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9d OCA], [https://pdbe.org/1w9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w9d RCSB], [https://www.ebi.ac.uk/pdbsum/1w9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w9d ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SEH:S-BENZYL+PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'>SEH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1myr|1myr]], [[1w9b|1w9b]]</div></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thioglucosidase Thioglucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.147 3.2.1.147] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9d OCA], [https://pdbe.org/1w9d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w9d RCSB], [https://www.ebi.ac.uk/pdbsum/1w9d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w9d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
| Line 16: | Line 19: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w9d ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w9d ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors. | ||
| + | |||
| + | The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors.,Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170<ref>PMID:15889170</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 1w9d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Sinapis alba]] |
| + | [[Category: Thioglucosidase]] | ||
| + | [[Category: Arzt, S]] | ||
| + | [[Category: Bourderioux, A]] | ||
| + | [[Category: Burmeister, W P]] | ||
| + | [[Category: Cottaz, S]] | ||
| + | [[Category: Gueyrard, D]] | ||
| + | [[Category: Lefoix, M]] | ||
| + | [[Category: Rollin, P]] | ||
| + | [[Category: Tatibouet, A]] | ||
| + | [[Category: Glucotropaeolin]] | ||
| + | [[Category: Glusosinolate]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Myrosinase]] | ||
| + | [[Category: Thio-glucoside]] | ||
| + | [[Category: Thioglycosidase]] | ||
| + | [[Category: Thiohydroximate]] | ||
Revision as of 09:17, 24 February 2021
S. alba myrosinase in complex with S-ethyl phenylacetothiohydroximate- O-sulfate
| |||||||||||
