1du0
From Proteopedia
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'''ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX''' | '''ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX''' | ||
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[[Category: Pabo, C O.]] | [[Category: Pabo, C O.]] | ||
[[Category: Rould, M A.]] | [[Category: Rould, M A.]] | ||
| - | [[Category: | + | [[Category: Dna-binding protein]] |
| - | [[Category: | + | [[Category: Homeodomain]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:16:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:16, 2 May 2008
ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX
Overview
We have determined the crystal structure of a complex containing the engrailed homeodomain Gln50 --> Ala variant (QA50) bound to the wild-type optimal DNA site (TAATTA) at 2.0 A resolution. Biochemical and genetic studies by other groups have suggested that residue 50 is an important determinant of differential DNA-binding specificity among homeodomains (distinguishing among various sites of the general form TAATNN). However, biochemical studies of the QA50 variant had revealed that it binds almost as tightly as the wild-type protein and with only modest changes in specificity. We have now determined the crystal structure of the QA50 variant to help understand the role of residue 50 in site-specific recognition. Our cocrystal structure shows some interesting changes in the water structure at the site of the substitution and shows some changes in the conformations of neighboring side chains. However, the structure, like the QA50 biochemical data, suggests that Gln50 plays a relatively modest role in determining the affinity and specificity of the engrailed homeodomain.
About this Structure
1DU0 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Exploring the role of glutamine 50 in the homeodomain-DNA interface: crystal structure of engrailed (Gln50 --> ala) complex at 2.0 A., Grant RA, Rould MA, Klemm JD, Pabo CO, Biochemistry. 2000 Jul 18;39(28):8187-92. PMID:10889025 Page seeded by OCA on Fri May 2 14:16:22 2008
