6x9z
From Proteopedia
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==De novo design of transmembrane beta-barrels== | ==De novo design of transmembrane beta-barrels== | ||
| - | <StructureSection load='6x9z' size='340' side='right'caption='[[6x9z]]' scene=''> | + | <StructureSection load='6x9z' size='340' side='right'caption='[[6x9z]], [[Resolution|resolution]] 2.05Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X9Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6x9z]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X9Z FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x9z OCA], [https://pdbe.org/6x9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x9z RCSB], [https://www.ebi.ac.uk/pdbsum/6x9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x9z ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x9z OCA], [https://pdbe.org/6x9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x9z RCSB], [https://www.ebi.ac.uk/pdbsum/6x9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x9z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transmembrane beta-barrel proteins (TMBs) are of great interest for single-molecule analytical technologies because they can spontaneously fold and insert into membranes and form stable pores, but the range of pore properties that can be achieved by repurposing natural TMBs is limited. We leverage the power of de novo computational design coupled with a "hypothesis, design, and test" approach to determine TMB design principles, notably, the importance of negative design to slow beta-sheet assembly. We design new eight-stranded TMBs, with no homology to known TMBs, that insert and fold reversibly into synthetic lipid membranes and have nuclear magnetic resonance and x-ray crystal structures very similar to the computational models. These advances should enable the custom design of pores for a wide range of applications. | ||
| + | |||
| + | De novo design of transmembrane beta barrels.,Vorobieva AA, White P, Liang B, Horne JE, Bera AK, Chow CM, Gerben S, Marx S, Kang A, Stiving AQ, Harvey SR, Marx DC, Khan GN, Fleming KG, Wysocki VH, Brockwell DJ, Tamm LK, Radford SE, Baker D Science. 2021 Feb 19;371(6531). pii: 371/6531/eabc8182. doi:, 10.1126/science.abc8182. PMID:33602829<ref>PMID:33602829</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6x9z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Baker D]] | + | [[Category: Baker, D]] |
| - | [[Category: Bera | + | [[Category: Bera, A K]] |
| - | [[Category: Kang | + | [[Category: Kang, A S]] |
| - | [[Category: Vorobieva | + | [[Category: Vorobieva, A A]] |
| + | [[Category: De novo design]] | ||
| + | [[Category: De novo protein]] | ||
| + | [[Category: Transmembrane beta-barrel]] | ||
Revision as of 14:57, 3 March 2021
De novo design of transmembrane beta-barrels
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