6x1k
From Proteopedia
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==Solution NMR structure of de novo designed TMB2.3== | ==Solution NMR structure of de novo designed TMB2.3== | ||
| - | <StructureSection load='6x1k' size='340' side='right'caption='[[6x1k]]' scene=''> | + | <StructureSection load='6x1k' size='340' side='right'caption='[[6x1k]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[6x1k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X1K FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x1k OCA], [https://pdbe.org/6x1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x1k RCSB], [https://www.ebi.ac.uk/pdbsum/6x1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x1k ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x1k OCA], [https://pdbe.org/6x1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x1k RCSB], [https://www.ebi.ac.uk/pdbsum/6x1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x1k ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transmembrane beta-barrel proteins (TMBs) are of great interest for single-molecule analytical technologies because they can spontaneously fold and insert into membranes and form stable pores, but the range of pore properties that can be achieved by repurposing natural TMBs is limited. We leverage the power of de novo computational design coupled with a "hypothesis, design, and test" approach to determine TMB design principles, notably, the importance of negative design to slow beta-sheet assembly. We design new eight-stranded TMBs, with no homology to known TMBs, that insert and fold reversibly into synthetic lipid membranes and have nuclear magnetic resonance and x-ray crystal structures very similar to the computational models. These advances should enable the custom design of pores for a wide range of applications. | ||
| + | |||
| + | De novo design of transmembrane beta barrels.,Vorobieva AA, White P, Liang B, Horne JE, Bera AK, Chow CM, Gerben S, Marx S, Kang A, Stiving AQ, Harvey SR, Marx DC, Khan GN, Fleming KG, Wysocki VH, Brockwell DJ, Tamm LK, Radford SE, Baker D Science. 2021 Feb 19;371(6531). pii: 371/6531/eabc8182. doi:, 10.1126/science.abc8182. PMID:33602829<ref>PMID:33602829</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6x1k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Baker D]] | + | [[Category: Synthetic construct sequences]] |
| - | [[Category: Chow | + | [[Category: Baker, D]] |
| - | [[Category: Liang B]] | + | [[Category: Chow, C M]] |
| - | [[Category: Tamm | + | [[Category: Liang, B]] |
| - | [[Category: Vorobieva | + | [[Category: Tamm, L K]] |
| + | [[Category: Vorobieva, A A]] | ||
| + | [[Category: Beta-barrel]] | ||
| + | [[Category: De novo design]] | ||
| + | [[Category: Membrane protein]] | ||
Revision as of 14:57, 3 March 2021
Solution NMR structure of de novo designed TMB2.3
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