6y4j
From Proteopedia
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==Engineered Fructosyl Peptide Oxidase== | ==Engineered Fructosyl Peptide Oxidase== | ||
| - | <StructureSection load='6y4j' size='340' side='right'caption='[[6y4j]]' scene=''> | + | <StructureSection load='6y4j' size='340' side='right'caption='[[6y4j]], [[Resolution|resolution]] 1.38Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4J OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6y4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y4J FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y4j OCA], [https://pdbe.org/6y4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y4j RCSB], [https://www.ebi.ac.uk/pdbsum/6y4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y4j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fructosyl peptide oxidases (FPOXs) are enzymes currently used in enzymatic assays to measure the concentration of glycated hemoglobin and albumin in blood samples, which serve as biomarkers of diabetes. However, since FPOX are unable to work directly on glycated proteins, current enzymatic assays are based on a preliminary proteolytic digestion of the target proteins. Herein, to improve the speed and costs of the enzymatic assays for diabetes testing, we applied a rational design approach to engineer a novel enzyme with a wider access tunnel to the catalytic site, using a combination of Rosetta design and molecular dynamics simulations. Our final design, L3_35A, shows a significantly wider and shorter access tunnel, resulting from the deletion of five-amino acids lining the gate structures and from a total of 35 point mutations relative to the wild-type (WT) enzyme. Indeed, upon experimental testing, our engineered enzyme shows good structural stability and maintains significant activity relative to the WT. | ||
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| + | Rational backbone redesign of a fructosyl peptide oxidase to widen its active site access tunnel.,Rigoldi F, Donini S, Torretta A, Carbone A, Redaelli A, Bandiera T, Parisini E, Gautieri A Biotechnol Bioeng. 2020 Aug 14. doi: 10.1002/bit.27535. PMID:32797625<ref>PMID:32797625</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6y4j" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus coli migula 1895]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Donini S]] | + | [[Category: Donini, S]] |
| - | [[Category: Gautieri A]] | + | [[Category: Gautieri, A]] |
| - | [[Category: Parisini E]] | + | [[Category: Parisini, E]] |
| - | [[Category: Rigoldi F]] | + | [[Category: Rigoldi, F]] |
| + | [[Category: Oxidoreductase]] | ||
Revision as of 14:58, 3 March 2021
Engineered Fructosyl Peptide Oxidase
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