2fhz

From Proteopedia

(Difference between revisions)

Revision as of 15:27, 3 March 2021

Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein

Structural highlights

2fhz is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2a8k
Gene:	imm ("Bacillus coli" Migula 1895), col ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IMM5_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE5 encoding colicin E5, against colicin E5. [CEA5_ECOLX] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This colicin is an endonuclease.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Colicin E5 is a tRNA-specific ribonuclease that recognizes and cleaves four tRNAs in Escherichia coli that contain the hypermodified nucleoside queuosine (Q) at the wobble position. Cells that produce colicin E5 also synthesize the cognate immunity protein (Im5) that rapidly and tightly associates with colicin E5 to prevent it from cleaving its own tRNAs to avoid suicide. We report here the crystal structure of Im5 in a complex with the activity domain of colicin E5 (E5-CRD) at 1.15A resolution. The structure reveals an extruded domain from Im5 that docks into the recessed RNA binding cleft in E5-CRD, resulting in extensive interactions between the two proteins. The interactions are primarily hydrophilic, with an interface that contains complementary surface charges between the two proteins. Detailed interactions in three separate regions of the interface account for specific recognition of colicin E5 by Im5. Furthermore, single-site mutational studies of Im5 confirmed the important role of particular residues in recognition and binding of colicin E5. Structural comparison of the complex reported here with E5-CRD alone, as well as with a docking model of RNA-E5-CRD, indicates that Im5 achieves its inhibition by physically blocking the cleft in colicin E5 that engages the RNA substrate.

Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity protein.,Luna-Chavez C, Lin YL, Huang RH J Mol Biol. 2006 Apr 28;358(2):571-9. Epub 2006 Feb 24. PMID:16524591^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luna-Chavez C, Lin YL, Huang RH. Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity protein. J Mol Biol. 2006 Apr 28;358(2):571-9. Epub 2006 Feb 24. PMID:16524591 doi:10.1016/j.jmb.2006.02.014

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fhz"

@@ Line 1: / Line 1: @@
 ==Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein==
-<StructureSection load='2fhz' size='340' side='right' caption='[[2fhz]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
+<StructureSection load='2fhz' size='340' side='right'caption='[[2fhz]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2fhz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FHZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2fhz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FHZ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a8k|2a8k]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a8k|2a8k]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">imm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), col ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">imm ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), col ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhz OCA], [http://pdbe.org/2fhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fhz RCSB], [http://www.ebi.ac.uk/pdbsum/2fhz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhz OCA], [https://pdbe.org/2fhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fhz RCSB], [https://www.ebi.ac.uk/pdbsum/2fhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IMM5_ECOLX IMM5_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE5 encoding colicin E5, against colicin E5. [[http://www.uniprot.org/uniprot/CEA5_ECOLX CEA5_ECOLX]] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This colicin is an endonuclease.
+[[https://www.uniprot.org/uniprot/IMM5_ECOLX IMM5_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE5 encoding colicin E5, against colicin E5. [[https://www.uniprot.org/uniprot/CEA5_ECOLX CEA5_ECOLX]] Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. This colicin is an endonuclease.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 31: @@
 ==See Also==
-*[[Colicin|Colicin]]
+*[[Colicin 3D structures|Colicin 3D structures]]
-*[[Colicin Immunity Protein|Colicin Immunity Protein]]
+*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
 == References ==
 <references/>
@@ Line 38: / Line 38: @@
 </StructureSection>
 [[Category: Bacillus coli migula 1895]]
+[[Category: Large Structures]]
 [[Category: Huang, R H]]
 [[Category: Lin, Y L]]

2fhz

From Proteopedia

Revision as of 15:27, 3 March 2021

Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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