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| | ==Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA== | | ==Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA== |
| - | <StructureSection load='2fo1' size='340' side='right' caption='[[2fo1]], [[Resolution|resolution]] 3.12Å' scene=''> | + | <StructureSection load='2fo1' size='340' side='right'caption='[[2fo1]], [[Resolution|resolution]] 3.12Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2fo1]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FO1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FO1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2fo1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FO1 FirstGlance]. <br> |
| | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LAG-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), sel-8, lag-3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), lin-12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LAG-1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), sel-8, lag-3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), lin-12 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fo1 OCA], [http://pdbe.org/2fo1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fo1 RCSB], [http://www.ebi.ac.uk/pdbsum/2fo1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fo1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fo1 OCA], [https://pdbe.org/2fo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fo1 RCSB], [https://www.ebi.ac.uk/pdbsum/2fo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fo1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LAG3_CAEEL LAG3_CAEEL]] Glp-1 and lin-12 promote signalling by recruiting lag-3 to target promoters, where it functions as a transcriptional activator. [[http://www.uniprot.org/uniprot/LIN12_CAEEL LIN12_CAEEL]] Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.<ref>PMID:3419531</ref> <ref>PMID:3000611</ref> | + | [[https://www.uniprot.org/uniprot/LAG3_CAEEL LAG3_CAEEL]] Glp-1 and lin-12 promote signalling by recruiting lag-3 to target promoters, where it functions as a transcriptional activator. [[https://www.uniprot.org/uniprot/LIN12_CAEEL LIN12_CAEEL]] Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.<ref>PMID:3419531</ref> <ref>PMID:3000611</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Caeel]] | | [[Category: Caeel]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Kovall, R A]] | | [[Category: Kovall, R A]] |
| | [[Category: Wilson, J J]] | | [[Category: Wilson, J J]] |
| Structural highlights
Function
[LAG3_CAEEL] Glp-1 and lin-12 promote signalling by recruiting lag-3 to target promoters, where it functions as a transcriptional activator. [LIN12_CAEEL] Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Notch signaling mediates communication between cells and is essential for proper embryonic patterning and development. CSL is a DNA binding transcription factor that regulates transcription of Notch target genes by interacting with coregulators. Transcriptional activation requires the displacement of corepressors from CSL by the intracellular portion of the receptor Notch (NotchIC) and the recruitment of the coactivator protein Mastermind to the complex. Here we report the 3.1 A structure of the ternary complex formed by CSL, NotchIC, and Mastermind bound to DNA. As expected, the RAM domain of Notch interacts with the beta trefoil domain of CSL; however, the C-terminal domain of CSL has an unanticipated central role in the interface formed with the Notch ankyrin repeats and Mastermind. Ternary complex formation induces a substantial conformational change within CSL, suggesting a molecular mechanism for the conversion of CSL from a repressor to an activator.
Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA.,Wilson JJ, Kovall RA Cell. 2006 Mar 10;124(5):985-96. PMID:16530045[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yochem J, Weston K, Greenwald I. The Caenorhabditis elegans lin-12 gene encodes a transmembrane protein with overall similarity to Drosophila Notch. Nature. 1988 Oct 6;335(6190):547-50. PMID:3419531 doi:http://dx.doi.org/10.1038/335547a0
- ↑ Greenwald I. lin-12, a nematode homeotic gene, is homologous to a set of mammalian proteins that includes epidermal growth factor. Cell. 1985 Dec;43(3 Pt 2):583-90. PMID:3000611
- ↑ Wilson JJ, Kovall RA. Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA. Cell. 2006 Mar 10;124(5):985-96. PMID:16530045 doi:10.1016/j.cell.2006.01.035
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