1dv0
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1dv0.gif|left|200px]] | [[Image:1dv0.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1dv0", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1dv0| PDB=1dv0 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Refined NMR solution structure of the C-terminal UBA domain of the human homologue of RAD23A (HHR23A)''' | '''Refined NMR solution structure of the C-terminal UBA domain of the human homologue of RAD23A (HHR23A)''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1DV0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry | + | 1DV0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uba 1uba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV0 OCA]. |
==Reference== | ==Reference== | ||
| Line 29: | Line 26: | ||
[[Category: Mueller, T D.]] | [[Category: Mueller, T D.]] | ||
[[Category: Withers-Ward, E S.]] | [[Category: Withers-Ward, E S.]] | ||
| - | [[Category: | + | [[Category: Helical bundle]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:18:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:18, 2 May 2008
Refined NMR solution structure of the C-terminal UBA domain of the human homologue of RAD23A (HHR23A)
Overview
The DNA repair protein HHR23A is a highly conserved protein that functions in nucleotide excision repair. HHR23A contains two ubiquitin associated domains (UBA) that are conserved in a number of proteins with diverse functions involved in ubiquitination, UV excision repair, and signaling pathways via protein kinases. The cellular binding partners of UBA domains remain unclear; however, we previously found that the HHR23A UBA(2) domain interacts specifically with the HIV-1 Vpr protein. Analysis of the low resolution solution structure of HHR23A UBA(2) revealed a hydrophobic loop region of the UBA(2) domain that we predicted was the interface for protein/protein interactions. Here we present results of in vitro binding studies that demonstrate the requirement of this hydrophobic loop region for interaction with human immunodeficiency virus (HIV-1) Vpr. A single point mutation of the Pro at residue 333 to a Glu totally abolishes the binding of HIV-1 Vpr to UBA(2). High resolution NMR structures of the binding deficient UBA(2) mutant P333E as well as of the wild-type UBA(2) domain were determined to compare the effect of this mutation on the structure. Small but significant differences are observed only locally at the site of the mutation. The biochemical and structural analysis confirms the function of the HHR23A UBA(2) GFP-loop as the protein/protein interacting domain.
About this Structure
1DV0 is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1uba. Full crystallographic information is available from OCA.
Reference
Biochemical and structural analysis of the interaction between the UBA(2) domain of the DNA repair protein HHR23A and HIV-1 Vpr., Withers-Ward ES, Mueller TD, Chen IS, Feigon J, Biochemistry. 2000 Nov 21;39(46):14103-12. PMID:11087358 Page seeded by OCA on Fri May 2 14:18:49 2008
