6vup
From Proteopedia
(Difference between revisions)
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==Reverse Transcriptase Diabody with R83T/E85C Mutations== | ==Reverse Transcriptase Diabody with R83T/E85C Mutations== | ||
| - | <StructureSection load='6vup' size='340' side='right'caption='[[6vup]]' scene=''> | + | <StructureSection load='6vup' size='340' side='right'caption='[[6vup]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VUP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6vup]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VUP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vup OCA], [https://pdbe.org/6vup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vup RCSB], [https://www.ebi.ac.uk/pdbsum/6vup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vup ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scFv ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vup OCA], [https://pdbe.org/6vup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vup RCSB], [https://www.ebi.ac.uk/pdbsum/6vup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vup ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This work presents a method for introducing synthetic symmetry into protein crystallization samples using an antibody fragment termed a diabody (Dab). These Dabs contain two target binding sites, and engineered disulfide bonds have been included to modulate Dab flexibility. The impacts of Dab engineering have been observed through assessment of thermal stability, small-angle X-ray scattering, and high-resolution crystal structures. Complexes between the engineered Dabs and HIV-1 reverse transcriptase (RT) bound to a high-affinity DNA aptamer were also generated to explore the capacity of engineered Dabs to enable the crystallization of bound target proteins. This strategy increased the crystallization hit frequency obtained for RT-aptamer, and the structure of a Dab-RT-aptamer complex was determined to 3.0-A resolution. Introduction of synthetic symmetry using a Dab could be a broadly applicable strategy, especially when monoclonal antibodies for a target have previously been identified. | ||
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| + | Co-crystallization with diabodies: a case study for the introduction of synthetic symmetry.,Chesterman C, Arnold E Structure. 2021 Feb 23. pii: S0969-2126(21)00045-9. doi:, 10.1016/j.str.2021.02.001. PMID:33636101<ref>PMID:33636101</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6vup" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Arnold E]] | + | [[Category: Arnold, E]] |
| - | [[Category: Chesterman C]] | + | [[Category: Chesterman, C]] |
| + | [[Category: Antibody fragment]] | ||
| + | [[Category: Protein binding]] | ||
Revision as of 17:51, 10 March 2021
Reverse Transcriptase Diabody with R83T/E85C Mutations
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