7ci3
From Proteopedia
(Difference between revisions)
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==The crystal structure of the SARS-CoV-2 ORF7a ectodomain== | ==The crystal structure of the SARS-CoV-2 ORF7a ectodomain== | ||
| - | <StructureSection load='7ci3' size='340' side='right'caption='[[7ci3]]' scene=''> | + | <StructureSection load='7ci3' size='340' side='right'caption='[[7ci3]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CI3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ci3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CI3 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ci3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ci3 OCA], [https://pdbe.org/7ci3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ci3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ci3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ci3 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ci3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ci3 OCA], [https://pdbe.org/7ci3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ci3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ci3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ci3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Dysregulated immune cell responses have been linked to the severity of coronavirus disease 2019 (COVID-19), but the specific viral factors of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were currently unknown. Herein, we reveal that the Immunoglobulin-like fold ectodomain of the viral protein SARS-CoV-2 ORF7a interacts with high efficiency to CD14(+) monocytes in human peripheral blood, compared to pathogenic protein SARS-CoV ORF7a. The crystal structure of SARS-CoV-2 ORF7a at 2.2 A resolution reveals three remarkable changes on the amphipathic side of the four-stranded beta-sheet, implying a potential functional interface of the viral protein. Importantly, SARS-CoV-2 ORF7a coincubation with CD14(+) monocytes ex vivo triggered a decrease in HLA-DR/DP/DQ expression levels and upregulated significant production of proinflammatory cytokines, including IL-6, IL-1beta, IL-8, and TNF-alpha. Our work demonstrates that SARS-CoV-2 ORF7a is an immunomodulating factor for immune cell binding and triggers dramatic inflammatory responses, providing promising therapeutic drug targets for pandemic COVID-19. | ||
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| + | Structural insight reveals SARS-CoV-2 ORF7a as an immunomodulating factor for human CD14(+) monocytes.,Zhou Z, Huang C, Zhou Z, Huang Z, Su L, Kang S, Chen X, Chen Q, He S, Rong X, Xiao F, Chen J, Chen S iScience. 2021 Mar 19;24(3):102187. doi: 10.1016/j.isci.2021.102187. Epub 2021, Feb 12. PMID:33615195<ref>PMID:33615195</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ci3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chen S]] | + | [[Category: Chen, S]] |
| - | [[Category: Zhou Z]] | + | [[Category: Zhou, Z]] |
| + | [[Category: Accessory protein]] | ||
| + | [[Category: Er membrane]] | ||
| + | [[Category: Ig-like fold]] | ||
| + | [[Category: Type-i transmembrane protein]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 18:03, 10 March 2021
The crystal structure of the SARS-CoV-2 ORF7a ectodomain
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