6vqq
From Proteopedia
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==CryoEM Structure of the Plasmodium falciparum transporter PfFNT== | ==CryoEM Structure of the Plasmodium falciparum transporter PfFNT== | ||
| - | <StructureSection load='6vqq' size='340' side='right'caption='[[6vqq]]' scene=''> | + | <StructureSection load='6vqq' size='340' side='right'caption='[[6vqq]], [[Resolution|resolution]] 2.56Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VQQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6vqq]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VQQ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqq OCA], [https://pdbe.org/6vqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vqq RCSB], [https://www.ebi.ac.uk/pdbsum/6vqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqq ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF3D7_0316600 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqq OCA], [https://pdbe.org/6vqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vqq RCSB], [https://www.ebi.ac.uk/pdbsum/6vqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The intra-erythrocyte stage of P. falciparum relies primarily on glycolysis to generate adenosine triphosphate (ATP) and the energy required to support growth and reproduction. Lactic acid, a metabolic byproduct of glycolysis, is potentially toxic as it lowers the pH inside the parasite. Plasmodium falciparum formate-nitrite transporter (PfFNT), a 34-kDa transmembrane protein, has been identified as a novel drug target as it exports lactate from inside the parasite to the surrounding parasitophorous vacuole within the erythrocyte cytosol. The structure and detailed molecular mechanism of this membrane protein are not yet available. Here we present structures of PfFNT in the absence and presence of the functional inhibitor MMV007839 at resolutions of 2.56 A and 2.78 A using single-particle cryo-electron microscopy. Genetic analysis and transport assay indicate that PfFNT is able to transfer lactate across the membrane. Combined, our data suggest a stepwise displacement mechanism for substrate transport. The PfFNT membrane protein is capable of picking up lactate ions from the parasite's cytosol, converting them to lactic acids and then exporting these acids into the extracellular space. | ||
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| + | Structural basis of transport and inhibition of the Plasmodium falciparum transporter PfFNT.,Lyu M, Su CC, Kazura JW, Yu EW EMBO Rep. 2021 Jan 20:e51628. doi: 10.15252/embr.202051628. PMID:33471955<ref>PMID:33471955</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6vqq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Lyu M]] | + | [[Category: Plaf7]] |
| - | [[Category: Su | + | [[Category: Lyu, M]] |
| + | [[Category: Su, C C]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Transporter]] | ||
Revision as of 06:53, 17 March 2021
CryoEM Structure of the Plasmodium falciparum transporter PfFNT
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